FN Archimer Export Format
PT J
TI Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source
BT 
AF de la Broise, Denis
   Ventura, Mariana
   Chauchat, Luc
   Guerreiro, Maurean
   Michez, Teo
   Vinet, Thibaud
   Gautron, Nicolas
   Le Grand, Fabienne
   Bideau, Antoine
   Goïc, Nelly Le
   Bidault, Adeline
   Lambert, Christophe
   Soudant, Philippe
AS 1:1;2:1;3:3;4:1;5:1;6:2;7:1;8:3;9:1;10:1;11:1;12:3;13:3;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;
C1 CNRS, IRD, Ifremer, Université de Bretagne Occidentale, UMR6539 LEMAR, F-29280 Plouzané, France
   DENITRAL, F-22400 Lamballe, France
   CNRS, IRD, Ifremer, Université de Bretagne Occidentale, UMR6539 LEMAR, F-29280 Plouzané, France
C2 UBO, FRANCE
   DENITRAL, FRANCE
   CNRS, FRANCE
UM LEMAR
IN WOS Cotutelle UMR
   DOAJ
   copubli-france
IF 5.4
TC 4
UR https://archimer.ifremer.fr/doc/00787/89890/95386.pdf
   https://archimer.ifremer.fr/doc/00787/89890/95387.zip
LA English
DT Article
DE ;Thraustochytrids;Aurantiochytrium;PUFA;DHA;digestate;oxygen;scale-up;pilot
AB The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA–rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of Aurantiochytrium mangrovei on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures. Pilot-scale reactors were airlift cylinders. Batch and fed-batch cultures were tested. Cultures over 38 to 62 h achieved a dry cell weight productivity of 3.3 to 5.5 g.L−1.day−1, and a substrate to biomass yield of up to 0.3. DHA productivity ranged from 10 to 0.18 mg.L−1.day−1. Biomass productivity appears linearly related to oxygen transfer rate. Bacterial contamination of cultures was low enough to avoid impacts on fatty acid composition of the biomass. A specific work on microbial risks assessment (in supplementary files) showed that the biomass can be securely used as feed. However, to date, there is a law void in EU legislation regarding the recycling of nitrogen from digestate from animal waste for microalgae biomass and its usage in animal feed. Overall, the proposed process appears similar to the industrial yeast production process (non-axenic heterotrophic process, dissolved oxygen supply limiting growth, similar cell size). Such similarity could help in further industrial developments. 
PY 2022
PD AUG
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 20
IS 8
UT 000846552800001
DI 10.3390/md20080499
ID 89890
ER

EF