FN Archimer Export Format
PT J
TI Structural variation turnovers and defective genomes: key drivers for the in vitro evolution of the large double-stranded DNA koi herpesvirus (KHV)
BT 
AF Fuandila, Nurul Novelia
   Gosselin-Grenet, Anne-Sophie
   Tilak, Marie-Ka
   Bergmann, Sven M
   Escoubas, Jean-Michel
   Klafack, Sandro
   Lusiastuti, Angela Mariana
   Yuhana, Munti
   Fiston-Lavier, Anna-Sophie
   Avarre, Jean-Christophe
   Cherif, Emira
AS 1:1;2:2;3:1;4:3;5:4;6:5;7:6;8:7;9:8;10:1;11:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;
C1 ISEM,UnivMontpellier, CNRS, IRD,Montpellier, France
   DGIMI,UnivMontpellier, INRAE,Montpellier, France
   Institute of Infectology, Friedrich-Loeffer-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany
   IHPE, Univ Montpellier, CNRS, Ifremer, UPVD, Montpellier, France
   Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffer-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany
   Fish Health Laboratory, Research Institute for Freshwater Aquaculture and Fisheries Extension, Bogor, Indonesia
   Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Indonesia
   Institut Universitaire de France (IUF), Paris, France
C2 UNIV MONTPELLIER, FRANCE
   UNIV MONTPELLIER, FRANCE
   FRIEDRICH LOEFFER INST, GERMANY
   UNIV MONTPELLIER, FRANCE
   FRIEDRICH LOEFFER INST, GERMANY
   RIFAFE, INDONESIA
   UNIV AGRI BOGOR, INDONESIA
   INST UNIV FRANCE, FRANCE
UM IHPE
IN DOAJ
TC 0
UR https://archimer.ifremer.fr/doc/00788/89971/95490.pdf
LA English
DT Article
AB Structural variations (SVs) constitute a significant source of genetic variability in virus genomes. Yet knowledge about SV variability and contribution to the evolutionary process in large double-stranded (ds)DNA viruses is limited. Cyprinid herpesvirus 3 (CyHV-3), also commonly known as koi herpesvirus (KHV), has the largest dsDNA genome within herpesviruses. This virus has become one of the biggest threats to common carp and koi farming, resulting in high morbidity and mortalities of fishes, serious environmental damage, and severe economic losses. A previous study analyzing CyHV-3 virulence evolution during serial passages onto carp cell cultures suggested that CyHV-3 evolves, at least in vitro, through an assembly of haplotypes that alternatively become dominant or under-represented. The present study investigates the SV diversity and dynamics in CyHV-3 genome during 99 serial passages in cell culture using, for the first time, ultra-deep whole-genome and amplicon-based sequencing. The results indicate that KHV polymorphism mostly involves SVs. These SVs display a wide distribution along the genome and exhibit high turnover dynamics with a clear bias towards inversion and deletion events. Analysis of the pathogenesis-associated ORF150 region in ten intermediate cell passages highlighted mainly deletion, inversion and insertion variations that deeply altered the structure of ORF150. Our findings indicate that SV turnovers and defective genomes represent key drivers in the viral population dynamics and in vitro evolution of KHV. Thus, the present study can contribute to the basic research needed to design safe live-attenuated vaccines, classically obtained by viral attenuation after serial passages in cell culture.  
PY 2022
PD JUN
SO Peer Community Journal. Section: Infections
SN 2804-3871
PU Centre Mersienne
VL 2
DI 10.24072/pcjournal.154
ID 89971
ER

EF