FN Archimer Export Format
PT J
TI Hybridization-based DNA biosensing with a limit of detection of 4 fM in 30 s using an electrohydrodynamic concentration module fabricated by grayscale lithography
BT 
AF Tijunelyte, Inga
   Teillet, Jeffrey
   Bruand, Paul
   Courson, Remi
   Lecestre, Aurélie
   Joseph, Pierre
   Bancaud, Aurélien
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;
FF 1:;2:;3:;4:;5:;6:;7:;
C1 CNRS, LAAS, 7 avenue du colonel Roche, F-31400 Toulouse, France
C2 CNRS, FRANCE
IF 3.2
TC 0
UR https://archimer.ifremer.fr/doc/00791/90254/96306.pdf
LA English
DT Article
AB Speeding up and enhancing the performances of nucleic acid biosensing technologies have remained drivers for innovation. Here, we optimize a fluorimetry-based technology for DNA detection based on the concentration of linear targets paired with probes. The concentration module consists of a microfluidic channel with the shape of a funnel in which we monitor a viscoelastic flow and a counter-electrophoretic force. We report that the technology performs better with a target longer than 100 nucleotides (nt) and a probe shorter than 30 nt. We also prove that the control of the funnel geometry in 2.5D using grayscale lithography enhances sensitivity by 100-fold in comparison to chips obtained by conventional photolithography. With these optimized settings, we demonstrate a limit of detection of 4 fM in 30 s and a detection range of more than five decades. This technology hence provides an excellent balance between sensitivity and time to result. 
PY 2022
PD JUN
SO Biomicrofluidics
SN 1932-1058
PU AIP Publishing
VL 16
IS 4
UT 000841273700001
DI 10.1063/5.0073542
ID 90254
ER

EF