FN Archimer Export Format
PT J
TI Carbonic anhydrase in the scleractinian coral Stylophora pistillata - Characterization, localization, and role in biomineralization
BT 
AF MOYA, Aurelie
   TAMBUTTE, Sylvie
   BERTUCCI, Anthony
   TAMBUTTE, Eric
   LOTTO, Severine
   VULLO, Daniela
   SUPURAN, Claudiu T.
   ALLEMAND, Denis
   ZOCCOLA, Didier
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:2;8:1;9:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;
C1 Centre Scientifique de Monaco, Avenue Saint-Martin, MC-98000 Principality of Monaco
   Dipartimento di Chimica, University of Florence, Via della Lastruccia, 3, Rm. 188, Polo Scientifico, 50019-Sesto Fiorentino (Firenze), Italy
C2 CTR SCI MONACO, MONACO
   UNIV FLORENCE, ITALY
IF 5.52
TC 201
UR https://archimer.ifremer.fr/doc/00792/90394/95897.pdf
LA English
DT Article
AB Carbonic anhydrases (CA) play an important role in biomineralization from invertebrates to vertebrates. Previous experiments have investigated the role of CA in coral calcification, mainly by pharmacological approaches. This study reports the molecular cloning, sequencing, and immunolocalization of a CA isolated from the scleractinian coral Stylophora pistillata, named STPCA. Results show that STPCA is a secreted form of alpha-CA, which possesses a CA catalytic function, similar to the secreted human CAVI. We localized this enzyme at the calico-blastic ectoderm level, which is responsible for the precipitation of the skeleton. This localization supports the role of STPCA in the calcification process. In symbiotic scleractinian corals, calcification is stimulated by light, a phenomenon called "light-enhanced calcification" (LEC). The mechanism by which symbiont photosynthesis stimulates calcification is still enigmatic. We tested the hypothesis that coral genes are differentially expressed under light and dark conditions. By real-time PCR, we investigated the differential expression of STPCA to determine its role in the LEC phenomenon. Results show that the STPCA gene is expressed 2-fold more during the dark than the light. We suggest that in the dark, up-regulation of the STPCA gene represents a mechanism to cope with night acidosis. 
PY 2008
PD SEP
SO Journal Of Biological Chemistry
SN 0021-9258
PU Amer Soc Biochemistry Molecular Biology Inc
VL 283
IS 37
UT 000259012700043
BP 25475
EP 25484
DI 10.1074/jbc.M804726200
ID 90394
ER

EF