FN Archimer Export Format
PT J
TI Identification and expression of microRNAs in european eels Anguilla anguilla from two natural sites with different pollution levels
BT 
AF BERTUCCI, Anthony
   PIERRON, Fabien
   YE, Tao
   GONZALEZ, Patrice
   COUTURE, Patrice
   BAUDRIMONT, Magalie
AS 1:1;2:1;3:2;4:1;5:3;6:1;
FF 1:;2:;3:;4:;5:;6:;
C1 Univ. Bordeaux, UMR EPOC CNRS 5805, 33615, Pessac, France
   IGBMC - CNRS UMR 7104 - Inserm U 964, 1 BP 10142, 67404, Illkirch Cedex, France
   Institut National de La Recherche Scientifique (INRS), Centre Eau Terre Environnement, 490 rue De La Couronne, Québec, QC, G1K 9A9, Canada
C2 UNIV BORDEAUX, FRANCE
   CNRS, FRANCE
   INRS, CANADA
IF 6.792
TC 4
UR https://archimer.ifremer.fr/doc/00792/90395/95973.pdf
LA English
DT Article
DE ;MicroRNA;Anguilla Anguilla;Aquatic ecotoxicology;Epigenetics;Biomarker
AB MicroRNAs (miRNAs) are a class of small non-coding RNA that control multiple biological processes through negative post-transcriptional regulation of gene expression. Recently a role of miRNAs in the response of aquatic organisms to environmental toxicants emerged. Toxicant-induced changes in miRNA expression might then represent novel biomarkers to evaluate the health status of these organisms. In this study, we aimed to identify the miRNA repertoire in the liver of the European eel Anguilla anguilla and to compare their differential expression between a polluted site located in the Gironde Estuary and a pristine site in Arcachon Bay (France). A total of 299 mature miRNAs were identified. In polluted water, 19 miRNAs were up-regulated and 22 were down-regulated. We predicted that these differentially expressed miRNAs could target 490 genes that were involved in ribosome biogenesis, response to hormones, response to chemical and chromatin modification. Moreover, we observed only few examples (29) of negative correlation between the expression levels of miRNAs and their targets suggesting that, in the system studied, miRNAs might not only regulate gene expression directly by degrading mRNA but also by inhibiting protein translation or by regulating other epigenetic processes. This study is the first example of in situ investigation of the role of miRNAs in the response of a fish species to water quality. Our findings provide new insights into the involvement of epigenetic mechanisms in the response of animals chronically exposed to pollution and pave the way for the utilization of miRNAs in aquatic ecotoxicology. 
PY 2019
PD JUN
SO Environmental Pollution
SN 0269-7491
PU Elsevier Sci Ltd
VL 250
UT 000471088200029
BP 274
EP 283
DI 10.1016/j.envpol.2019.04.029
ID 90395
ER

EF