FN Archimer Export Format PT J TI Transcriptome Analysis of the Scleractinian Coral Stylophora pistillata BT AF KARAKO-LAMPERT, Sarit ZOCCOLA, Didier SALMON-DIVON, Mali KATZENELLENBOGEN, Mark TAMBUTTE, Sylvie BERTUCCI, Anthony HOEGH-GULDBERG, Ove DELEURY, Emeline ALLEMAND, Denis LEVY, Oren AS 1:1;2:2;3:3;4:1;5:2;6:2,4,5;7:6;8:4,7;9:2;10:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:; C1 Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, Ramat Gan, Israel. Ctr Sci Monaco, Monaco, Monaco. Ariel Univ, Dept Mol Biol, Ariel, Israel. Univ Nice Sophia Antipolis, UFR Sci, F-06189 Nice, France. James Cook Univ, Ctr Excellence Coral Reef Studies, Australian Res Council, Townsville, Qld 4811, Australia. Univ Queensland, Global Change Inst, St Lucia, Qld, Australia. Inst Sophia Agrobiotech INRA 1355, CNRS 7254, Sophia Antipolis, France. C2 UNIV BAR ILAN, ISRAEL CTR SCI MONACO, MONACO UNIV ARIEL, ISRAEL UNIV NICE, FRANCE UNIV JAMES COOK, AUSTRALIA UNIV QUEENSLAND, AUSTRALIA INRA, FRANCE IF 3.234 TC 44 UR https://archimer.ifremer.fr/doc/00792/90403/95906.pdf LA English DT Article AB The principal architects of coral reefs are the scleractinian corals; these species are divided in two major clades referred to as "robust'' and "complex'' corals. Although the molecular diversity of the "complex'' clade has received considerable attention, with several expressed sequence tag (EST) libraries and a complete genome sequence having been constructed, the "robust'' corals have received far less attention, despite the fact that robust corals have been prominent focal points for ecological and physiological studies. Filling this gap affords important opportunities to extend these studies and to improve our understanding of the differences between the two major clades. Here, we present an EST library from Stylophora pistillata (Esper 1797) and systematically analyze the assembled transcripts compared to putative homologs from the complete proteomes of six well-characterized metazoans: Nematostella vectensis, Hydra magnipapillata, Caenorhabditis elegans, Drosophila melanogaster, Strongylocentrotus purpuratus, Ciona intestinalis and Homo sapiens. Furthermore, comparative analyses of the Stylophora pistillata ESTs were performed against several Cnidaria from the Scleractinia, Actiniaria and Hydrozoa, as well as against other stony corals separately. Functional characterization of S. pistillata transcripts into KOG/COG categories and further description of Wnt and bone morphogenetic protein (BMP) signaling pathways showed that the assembled EST library provides sufficient data and coverage. These features of this new library suggest considerable opportunities for extending our understanding of the molecular and physiological behavior of "robust'' corals. PY 2014 PD FEB SO Plos One SN 1932-6203 PU Public Library Science VL 9 IS 2 UT 000331266000046 DI 10.1371/journal.pone.0088615 ID 90403 ER EF