FN Archimer Export Format
PT J
TI Coral Carbonic Anhydrases: Regulation by Ocean Acidification
BT 
AF ZOCCOLA, Didier
   INNOCENTI, Alessio
   BERTUCCI, Anthony
   TAMBUTTE, Eric
   SUPURAN, Claudiu T.
   TAMBUTTE, Sylvie
AS 1:1,2;2:3;3:1;4:1,2;5:3;6:1,2;
FF 1:;2:;3:;4:;5:;6:;
C1 Marine Biology Department, Centre Scientifique de Monaco, 8 Quai Antoine 1°, 98 000 Monaco, Monaco
   Laboratoire International Associé 647 BIOSENSIB, Centre Scientifique de Monaco-Centre National de la Recherche Scientifique, 8 Quai Antoine 1°, 98 000 Monaco, Monaco
   Neurofarba Department, University of Florence, Via Ugo Schiff 6, Polo Scientifico, Sesto Fiorentino, 50019 Firenze, Italy
C2 CTR SCI MONACO, MONACO
   CNRS, MONACO
   UNIV FLORENCE, ITALY
IN DOAJ
TC 33
UR https://archimer.ifremer.fr/doc/00792/90404/95920.pdf
LA English
DT Article
DE ;coral;calcification;ocean acidification;carbonic anhydrase;gene expression;enzyme activity;temperature;pH
AB Global change is a major threat to the oceans, as it implies temperature increase and acidification. Ocean acidification (OA) involving decreasing pH and changes in seawater carbonate chemistry challenges the capacity of corals to form their skeletons. Despite the large number of studies that have investigated how rates of calcification respond to ocean acidification scenarios, comparatively few studies tackle how ocean acidification impacts the physiological mechanisms that drive calcification itself. The aim of our paper was to determine how the carbonic anhydrases, which play a major role in calcification, are potentially regulated by ocean acidification. For this we measured the effect of pH on enzyme activity of two carbonic anhydrase isoforms that have been previously characterized in the scleractinian coral Stylophora pistillata. In addition we looked at gene expression of these enzymes in vivo. For both isoforms, our results show (1) a change in gene expression under OA (2) an effect of OA and temperature on carbonic anhydrase activity. We suggest that temperature increase could counterbalance the effect of OA on enzyme activity. Finally we point out that caution must, thus, be taken when interpreting transcriptomic data on carbonic anhydrases in ocean acidification and temperature stress experiments, as the effect of these stressors on the physiological function of CA will depend both on gene expression and enzyme activity. 
PY 2016
PD JUL
SO Marine Drugs
PU Mdpi
VL 14
IS 6
UT 000378770300006
DI 10.3390/md14060109
ID 90404
ER

EF