FN Archimer Export Format
PT J
TI Emergence of a Novel Lineage and Wide Spread of a blaCTX-M-15/IncHI2/ST1 Plasmid among Nosocomial Enterobacter in Guadeloupe
BT 
AF Pot, Matthieu
   REYNAUD, Yann
   Couvin, David
   Dereeper, Alexis
   Ferdinand, Séverine
   Bastian, Sylvaine
   Foucan, Tania
   Pommier, Jean-David
   Valette, Marc
   Talarmin, Antoine
   Guyomard-Rabenirina, Stéphanie
   Breurec, Sébastien
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;8:4;9:4;10:1;11:1;12:1,5,6;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;
C1 Transmission, Reservoir and Diversity of Pathogens Unit, Pasteur Institute of Guadeloupe, 97139 Les Abymes, France
   Laboratory of Clinical Microbiology, University Hospital Center of Guadeloupe, 97159 Pointe-à-Pitre, France
   Operational Hygiene Team, University Hospital Center of Guadeloupe, 97159 Pointe-à-Pitre, France
   Division of Intensive Care, University Hospital Center of Guadeloupe, 97159 Pointe-à-Pitre, France
   Faculty of Medicine Hyacinthe Bastaraud, University of the Antilles, 97157 Pointe-à-Pitre, France
   INSERM, Center for Clinical Investigation 1424, 97139 Les Abymes, France
C2 INST PASTEUR, FRANCE
   CHU POINTE A PITRE, FRANCE
   CHU POINTE A PITRE, FRANCE
   CHU POINTE A PITRE, FRANCE
   UNIV ANTILLES, FRANCE
   INSERM, FRANCE
IN DOAJ
IF 4.8
TC 1
UR https://archimer.ifremer.fr/doc/00799/91098/96741.pdf
LA English
DT Article
DE ;Caribbean;Enterobacter cloacae complex;ESBL;healthcare;hsp60;molecular sequencing;Nanopore;plasmid;ST114;ST1503
AB Between April 2018 and August 2019, a total of 135 strains of Enterobacter cloacae complex (ECC) were randomly collected at the University Hospital Center of Guadeloupe to investigate the structure and diversity of the local bacterial population. These nosocomial isolates were initially identified genetically by the hsp60 typing method, which revealed the clinical relevance of E. xiangfangensis (n = 69). Overall, 57/94 of the third cephalosporin-resistant strains were characterized as extended-spectrum-β-lactamase (ESBL) producers, and their whole-genome was sequenced using Illumina technology to determine the clonal relatedness and diffusion of resistance genes. We found limited genetic diversity among sequence types (STs). ST114 (n = 13), ST1503 (n = 9), ST53 (n = 5) and ST113 (n = 4), which belong to three different Enterobacter species, were the most prevalent among the 57 ESBL producers. The blaCTXM-15 gene was the most prevalent ESBL determinant (56/57) and was in most cases associated with IncHI2/ST1 plasmid replicon carriage (36/57). To fully characterize this predominant blaCTXM-15/IncHI2/ST1 plasmid, four isolates from different lineages were also sequenced using Oxford Nanopore sequencing technology to generate long-reads. Hybrid sequence analyses confirmed the circulation of a well-conserved plasmid among ECC members. In addition, the novel ST1503 and its associated species (ECC taxon 4) were analyzed, in view of its high prevalence in nosocomial infections. These genetic observations confirmed the overall incidence of nosocomial ESBL Enterobacteriaceae infections acquired in this hospital during the study period, which was clearly higher in Guadeloupe (1.59/1000 hospitalization days) than in mainland France (0.52/1,000 hospitalization days). This project revealed issues and future challenges for the management and surveillance of nosocomial and multidrug-resistant Enterobacter in the Caribbea 
PY 2022
PD OCT
SO Antibiotics-basel
SN 2079-6382
PU MDPI
VL 11
IS 10
UT 000872087000001
DI 10.3390/antibiotics11101443
ID 91098
ER

EF