FN Archimer Export Format
PT J
TI Sub-micrometric spatial distribution of amorphous and crystalline carbonates in biogenic crystals using coherent Raman microscopy
BT 
AF Dicko, Hamadou
   Grünewald, Tilman A.
   Ferrand, Patrick
   Vidal-Dupiol, Jeremie
   Teaniniuraitemoana, Vaihiti
   SHAM KOUA, Manaarii
   LE MOULLAC, Gilles
   Le Luyer, Jeremy
   SAULNIER, Denis
   Chamard, Virginie
   Duboisset, Julien
AS 1:1;2:1;3:1;4:2;5:3;6:3;7:3;8:3;9:3;10:1;11:1;
FF 1:;2:;3:;4:PDG-RBE-IHPE;5:PDG-RBE-RMPF;6:PDG-RBE-RMPF;7:PDG-RBE-RMPF;8:PDG-RBE-RMPF;9:PDG-RBE-RMPF;10:;11:;
C1 Aix-Marseille Univ, CNRS, Centrale Marseille, Institut Fresnel, Marseille, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, France
   Ifremer, UMR 241 Environnement Insulaire Océanien (EIO), Labex Corail, Centre du Pacifique, BP 49, Vairao 98719, French Polynesia
C2 UNIV AIX-MARSEILLE, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI MONTPELLIER
   TAHITI
SE PDG-RBE-IHPE
   PDG-RBE-RMPF
UM IHPE
   EIO
IN WOS Ifremer UMR
   copubli-france
   copubli-univ-france
IF 3
TC 3
UR https://archimer.ifremer.fr/doc/00800/91212/96940.pdf
LA English
DT Article
DE ;Coherent Raman;Optical microscopy;Calcium carbonate;Shell oyster;Crystalline transition;Biomineralization
AB In living organisms, calcium carbonate biomineralization combines complex bio-controlled physical and chemical processes to produce crystalline hierarchical hard tissues (usually calcite or aragonite) typically from an amorphous precursor phase. Understanding the nature of the successive transient amorphous phases potentially involved in the amorphous-to-crystalline transition requires characterization tools, which are able to provide a spatial and spectroscopic analysis of the biomineral structure. In this work, we present a highly sensitive coherent Raman microscopy approach, which allows one to image molecular bond concentrations in post mortem shells and living animals, by exploiting the vibrational signature of the different carbonates compounds. To this end, we target the calcium carbonate vibration mode and produce spatially and spectroscopically resolved images of the shell border of a mollusk shell, the Pinctada margaritifera pearl oyster. A novel approach is further presented to efficiently compare the amount of amorphous carbonate with respect to its crystalline counterpart. Finally, the whole microscopy method is used to image in vivo the shell border and demonstrate the feasibility and the reproducibility of the technique. These findings open chemical imaging perspectives for the study of biogenic and bio-inspired crystals. 
PY 2022
PD DEC
SO Journal Of Structural Biology
SN 1047-8477
PU Elsevier BV
VL 214
IS 4
UT 000890812500005
DI 10.1016/j.jsb.2022.107909
ID 91212
ER

EF