FN Archimer Export Format
PT J
TI Strong population genomic structure of the toxic dinoflagellate Alexandrium minutum inferred from meta‐transcriptome samples
BT 
AF le Gac, Mickael
   Mary, Lou
   Metegnier, Gabriel
   Quéré, Julien
   Siano, Raffaele
   Rodríguez, Francisco
   Destombe, Christophe
   Sourisseau, Marc
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;8:1;
FF 1:PDG-ODE-DYNECO-PELAGOS;2:PDG-ODE-DYNECO-PELAGOS;3:PDG-ODE-DYNECO-PELAGOS;4:PDG-ODE-DYNECO-PELAGOS;5:PDG-ODE-DYNECO-PELAGOS;6:;7:;8:PDG-ODE-DYNECO-PELAGOS;
C1 Ifremer, Dyneco, Plouzané, France
   Instituto Español de Oceanografía (IEO, CSIC),Vigo ,Spain
   Station Biologique de Roscoff, IRL 3614, CNRS, Sorbonne Université, Roscoff ,France
C2 IFREMER, FRANCE
   IEO, SPAIN
   UNIV SORBONNE, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer UPR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 5.1
TC 1
UR https://archimer.ifremer.fr/doc/00800/91219/96948.pdf
LA English
DT Article
AB Despite theoretical expectations, marine microeukaryote population are often highly structured and the mechanisms behind such patterns remain to be elucidated. These organisms display huge census population sizes, yet genotyping usually requires clonal strains originating from single cells, hindering proper population sampling. Estimating allelic frequency directly from population wide samples, without any isolation step, offers an interesting alternative. Here we validate the use of meta-transcriptome environmental samples to determine the population genetic structure of the dinoflagellate Alexandrium minutum. Strain and meta-transcriptome based results both indicated a strong genetic structure for A. minutum in Western Europe, to the level expected between cryptic species. The presence of numerous private alleles, and even fixed polymorphism, would indicate ancient divergence and absence of gene flow between populations. Single Nucleotide Polymorphisms (SNPs) displaying strong allele frequency differences were distributed throughout the genome, which might indicate pervasive selection from standing genetic variation (soft selective sweeps). However, a few genomic regions displayed extremely low diversity that could result from the fixation of adaptive de novo mutations (hard selective sweeps) within the populations. 
PY 2022
PD DEC
SO Environmental Microbiology
SN 1462-2912
PU Wiley
VL 24
IS 12
UT 000880524200001
BP 5966
EP 5983
DI 10.1111/1462-2920.16257
ID 91219
ER

EF