FN Archimer Export Format
PT J
TI Deciphering interactions between the marine dinoflagellate Prorocentrum lima and the fungus Aspergillus pseudoglaucus
BT 
AF Berry, Olivier
   Briand, Enora
   Bagot, Alizé
   Chaigné, Maud
   Meslet‐Cladière, Laurence
   Wang, Julien
   Grovel, Olivier
   Jansen, Jeroen J.
   Ruiz, Nicolas
   Robiou du Pont, Thibaut
   Pouchus, Yves François
   Hess, Philipp
   Bertrand, Samuel
AS 1:1;2:2;3:1,2;4:1,2;5:3;6:1;7:1;8:4;9:1;10:1;11:1;12:2;13:1;
FF 1:;2:PDG-ODE-PHYTOX-GENALG;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:PDG-ODE-PHYTOX;13:;
C1 Institut des Substances et Organismes de la Mer, ISOMER Nantes Université, UR 2160, Nantes, France
   IFREMER, PHYTOX, Nantes, France
   Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité et Écologie Microbienne, Plouzané, France
   Radboud University, Institute for Molecules and Materials, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands
C2 UNIV NANTES, FRANCE
   IFREMER, FRANCE
   UBO, FRANCE
   UNIV RADBOUD NIJMEGEN, NETHERLANDS
SI NANTES
SE PDG-ODE-PHYTOX-GENALG
   PDG-ODE-PHYTOX
IN WOS Ifremer UPR
   copubli-france
   copubli-europe
   copubli-univ-france
IF 5.1
TC 5
UR https://archimer.ifremer.fr/doc/00801/91309/97095.pdf
   https://archimer.ifremer.fr/doc/00801/91309/97096.pdf
LA English
DT Article
AB The comprehension of microbial interactions is one of the key challenges in marine microbial ecology. This study focused on exploring chemical interactions between the toxic dinoflagellate Prorocentrum lima and a filamentous fungal species, Aspergillus pseudoglaucus, which has been isolated from the microalgal culture. Such interspecies interactions are expected to occur even though they were rarely studied. Here, a co-culture system was designed in a dedicated microscale marine-like condition. This system allowed to explore microalgal-fungal physical and metabolic interactions in presence and absence of the bacterial consortium. Microscopic observation showed an unusual physical contact between the fungal mycelium and dinoflagellate cells. To delineate specialized metabolome alterations during microalgal-fungal co-culture metabolomes were monitored by high-performance liquid chromatography coupled to high-resolution mass spectrometry. In-depth multivariate statistical analysis using dedicated approaches highlighted (1) the metabolic alterations associated with microalgal-fungal co-culture, and (2) the impact of associated bacteria in microalgal metabolome response to fungal interaction. Unfortunately, only a very low number of highlighted features were fully characterised. However, an up-regulation of the dinoflagellate toxins okadaic acid and dinophysistoxin 1 was observed during co-culture in supernatants. Such results highlight the importance to consider microalgal-fungal interactions in the study of parameters regulating toxin production. 
PY 2023
PD FEB
SO Environmental Microbiology
SN 1462-2912
PU Wiley
VL 25
IS 2
UT 000889257300001
BP 250
EP 267
DI 10.1111/1462-2920.16271
ID 91309
ER

EF