Evaluation of Aurantiochytrium mangrovei Biomass Grown on Digestate as a Sustainable Feed Ingredient of Sea Bass, Dicentrarchus labrax, Juveniles and Larvae

Type Article
Date 2022-11
Language English
Author(s) Soudant Philippe5, Ventura Mariana1, Chauchat Luc1, Guerreiro Mauean1, Mathieu-Resuge Margaux, Le Grand Fabienne5, Simon VictorORCID4, Collet Sophie4, Zambonino Infante Jose-LuisORCID4, Le Goïc Nelly1, Lambert Christophe5, Fernandes Fleuriane2, Sillkina Alla2, Fernandes De Souza Marcella3, de La Broise Denis1
Affiliation(s) 1 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
2 : AlgaeWales Research Group, Centre for Sustainable Aquatic Research (CSAR), College of Science, Bioscience Department, Swansea University, Singleton Park, Swansea SA2 8PP, UK
3 : Department of Green Chemistry & Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
4 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
5 : Univ Brest, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France
Source Sustainability (2071-1050) (MDPI), 2022-11 , Vol. 14 , N. 21 , P. 14573 (23p.)
DOI 10.3390/su142114573
Note This article belongs to the Special Issue Marine Biotechnology for Sustainability of Ecologically Significant Resources
Keyword(s) aquaculture, nutrition, sustainability, n-3 long-chain polyunsaturated fatty acids, Thraustochytrids, microalgae, fish oil
Abstract

The use of microalgae as a sustainable source of n-3 long-chain polyunsaturated fatty acids (LC-PUFA) as an alternative to fish oils from small pelagic fish (e.g., anchovy, sardine) has received growing interest in the past few years. The present study aimed to: (i) produce Aurantiochytrium mangrovei biomass by heterotrophic fermentation using a medium containing anaerobic digestion liquid effluent, and (ii) evaluate a biomass rich in n-3 LC-PUFA and good quality proteins as a feed ingredient for sea bass juveniles and larvae. Two 800 L bioreactors were used to produce Aurantiochytrium biomass in non-axenic conditions. Biomass was then filtered through a crossflow filtration system (300 Kda ceramic membrane) and freeze-dried. Sea bass juveniles (32.7 ± 4.2 g) were fed both a control diet and a diet containing 15% of freeze-dried A. mangrovei biomass for 38 days. Juvenile survival percentage was 90% on average in both dietary conditions. Similar growth was observed between fish fed with both diets, demonstrating the feasibility to replace 15% of a standard fish feed by Aurantiochytrium biomass. The liver of sea bass juveniles fed with the A. mangrovei diet contained significantly higher proportions of 22:6n-3, 22:5n-6, and 20:4n-6 than those fed with the control diet, while the proportions of 16:0, 16:1n-7, and 18:1n-9 were significantly lower. The secondary oxidation, as measured by malonylaldehyde (MDA) content, in the liver and muscle of juveniles fed with the microalgae diet tended to be higher than in fish fed the control diet, but the differences were not statistically significant. Although the larvae survival percentage was low for all the tanks after 41 days of rearing, the inclusion of 15% of hydrolyzed A. mangrovei biomass in the larvae micro-diet did not impair the development of sea bass larvae and only marginally affected their lipid composition. In the future, we have to further optimize a sustainable workflow between Aurantiochytrium cultivation and fish feed production and confirm the zootechnical and biochemical results.

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Soudant Philippe, Ventura Mariana, Chauchat Luc, Guerreiro Mauean, Mathieu-Resuge Margaux, Le Grand Fabienne, Simon Victor, Collet Sophie, Zambonino Infante Jose-Luis, Le Goïc Nelly, Lambert Christophe, Fernandes Fleuriane, Sillkina Alla, Fernandes De Souza Marcella, de La Broise Denis (2022). Evaluation of Aurantiochytrium mangrovei Biomass Grown on Digestate as a Sustainable Feed Ingredient of Sea Bass, Dicentrarchus labrax, Juveniles and Larvae. Sustainability, 14(21), 14573 (23p.). Publisher's official version : https://doi.org/10.3390/su142114573 , Open Access version : https://archimer.ifremer.fr/doc/00801/91331/