FN Archimer Export Format
PT J
TI Genetic association of toxin production in the dinoflagellate Alexandrium minutum
BT 
AF Mary, Lou
   Quere, Julien
   Latimier, Marie
   Rovillon, Georges-Augustin
   Hégaret, Helene
   Réveillon, Damien
   Le Gac, Mickael
AS 1:1,2,3;2:1;3:1;4:2;5:3;6:2;7:1;
FF 1:PDG-ODE-DYNECO-PELAGOS;2:PDG-ODE-DYNECO-PELAGOS;3:PDG-ODE-DYNECO-PELAGOS;4:PDG-ODE-DYNECO-PHYC;5:;6:PDG-ODE-PHYTOX-METALG;7:PDG-ODE-DYNECO-PELAGOS;
C1 Ifremer, DYNECO PELAGOS, 29280 Plouzané, France
   Ifremer, PHYTOX, Laboratoire METALG, F-44000 Nantes, France
   Laboratoire des Sciences de l’Environnement Marin (LEMAR), UMR 6539 CNRS UBO IRD IFREMER - Institut Universitaire Européen de la Mer, 29280 Plouzané, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
SI BREST
   NANTES
SE PDG-ODE-DYNECO-PELAGOS
   PDG-ODE-DYNECO-PHYC
   PDG-ODE-PHYTOX-METALG
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
IF 3.9
TC 1
UR https://archimer.ifremer.fr/doc/00801/91345/97173.pdf
   https://archimer.ifremer.fr/doc/00801/91345/97174.pdf
LA English
DT Article
DE ;Alexandrium minutum;Genetic linkage map;Paralytic Shellfish Toxins (PSTs);sxtG;sxtI;Toxin inheritance
AB Dinoflagellates of the genus Alexandrium are responsible for harmful algal blooms and produce paralytic shellfish toxins (PSTs). Their very large and complex genomes make it challenging to identify the genes responsible for toxin synthesis. A family-based genomic association study was developed to determine the inheritance of toxin production in Alexandrium minutum and identify genomic regions linked to this production. We show that the ability to produce toxins is inheritable in a Mendelian way, while the heritability of the toxin profile is more complex. We developed the first dinoflagellate genetic linkage map. Using this map, several major results were obtained: 1. A genomic region related to the ability to produce toxins was identified. 2. This region does not contain any polymorphic sxt genes, known to be involved in toxin production in cyanobacteria. 3. The sxt genes, known to be present in a single cluster in cyanobacteria, are scattered on different linkage groups in A. minutum. 4. The expression of two sxt genes not assigned to any linkage group, sxtI and sxtG, may be regulated by the genomic region related to the ability to produce toxins. Our results provide new insights into the organization of toxicity-related genes in A. minutum, suggesting a dissociated genetic mechanism for the production of the different analogues and the ability to produce toxins. However, most of the newly identified genes remain unannotated. This study therefore proposes new candidate genes to be further explored to understand how dinoflagellates synthesize their toxins. 
PY 2022
PD NOV
SO Microbial Genomics
SN 2057-5858
PU Microbiology Society
VL 8
IS 11
UT 000993252000001
DI 10.1099/mgen.0.000879
ID 91345
ER

EF