FN Archimer Export Format
PT J
TI Genomic Diversity of Campylobacter lari Group Isolates from Europe and Australia in a One Health Context
BT 
AF Gourmelon, Michele
   Boukerb, Amine M.
   Nabi, Nesrine
   Banerji, Sangeeta
   Joensen, Katrine G.
   Serghine, Joelle
   Cormier, Alexandre
   Megraud, Francis
   Lehours, Philippe
   Alter, Thomas
   Ingle, Danielle J.
   Kirk, Martyn D.
   Nielsen, Eva M.
AS 1:1;2:1,2;3:1;4:3;5:4;6:1;7:5;8:6;9:6,7;10:8;11:9,10;12:9;13:4;
FF 1:PDG-RBE-SGMM-LSEM;2:;3:PDG-RBE-SGMM-LSEM;4:;5:;6:PDG-RBE-SGMM-LSEM;7:PDG-IRSI-SEBIMER;8:;9:;10:;11:;12:;13:;
C1 Ifremer, RBE-SGMM-LSEM, Plouzané, France
   University of Rouen Normandy, Laboratory of Microbiology Bacterial Communication and Anti-infectious Strategies (CBSA), Evreux, France
   Robert Koch Institute, Wernigerode, Germany
   Statens Serum Institut, Copenhagen, Denmark
   IFREMER, IRSI-SeBiMER, Plouzané, France
   BRIC Bordeaux Institute of Oncology, BRIC U1312, INSERM, University of Bordeaux, Bordeaux Hospital, Bordeaux, France
   French National Reference Centre for Campylobacters and Helicobacters, Bordeaux Hospital, Bordeaux, France
   Freie Universität Berlin, Berlin, Germany
   National Centre for Epidemiology and Population Health, Australian National University, Canberra, Australia
   Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia
C2 IFREMER, FRANCE
   UNIV ROUEN, FRANCE
   ROBERT KOCH INST, GERMANY
   STATENS SERUM INST, DENMARK
   IFREMER, FRANCE
   INSERM, FRANCE
   HOP BORDEAUX, FRANCE
   UNIV FREE BERLIN, GERMANY
   UNIV AUSTRALIAN NATL, AUSTRALIA
   UNIV MELBOURNE, AUSTRALIA
SI BREST
SE PDG-RBE-SGMM-LSEM
   PDG-IRSI-SEBIMER
IN WOS Ifremer UPR
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 4.4
TC 7
UR https://archimer.ifremer.fr/doc/00804/91613/97594.pdf
   https://archimer.ifremer.fr/doc/00804/91613/97595.pdf
   https://archimer.ifremer.fr/doc/00804/91613/97596.xlsx
LA English
DT Article
DE ;Campylobacter lad group;whole-genome sequencing;virulence genes;genomic diversity;One Health
AB Members of the Campylobacter lari group are causative agents of human gastroenteritis and are frequently found in shellfish, marine waters, shorebirds, and marine mammals. Within a One Health context, we used comparative genomics to characterize isolates from a diverse range of sources and geographical locations within Europe and Australia and assess possible transmission of food, animal, and environmental isolates to the human host. A total of 158 C. lari isolates from Australia, Denmark, France, and Germany, which included 82 isolates from human stool and blood, 12 from food, 14 from domestic animal, 19 from waterbirds, and 31 from the environment were analyzed. Genome-wide analysis of the genetic diversity, virulence, and antimicrobial resistance (AMR) traits was carried-out. Most of the isolates belonged to C. lari subsp. lari (Cll; 98, 62.0%), while C. lari subsp. concheus and C. lari urease-positive thermotolerant Campylobacter (UPTC) were represented by 12 (7.6%) and 15 (9.5%) isolates, respectively. Furthermore, 33 (20.9%) isolates were not assigned a subspecies and were thus attributed to distant Campylobacter spp. clades. Whole-genome sequence-derived multilocus sequence typing (MLST) and core-genome MLST (cgMLST) analyses revealed a high genetic diversity with 97 sequence types (STs), including 60 novel STs and 14 cgMLST clusters (≤10 allele differences), respectively. The most prevalent STs were ST-21, ST-70, ST-24, and ST-58 (accounting for 13.3%, 4.4%, 3.8%, and 3.2% of isolates, respectively). A high prevalence of the 125 examined virulence-related loci (from 76.8 to 98.4% per isolate) was observed, especially in Cll isolates, suggesting a probable human pathogenicity of these strains. IMPORTANCE Currently, relatedness between bacterial isolates impacting human health is easily monitored by molecular typing methods. These approaches rely on discrete loci or whole-genome sequence (WGS) analyses. Campylobacter lari is an emergent human pathogen isolated from diverse ecological niches, including fecal material from humans and animals, aquatic environments, and seafood. The presence of C. lari in such diverse sources underlines the importance of adopting an integrated One Health approach in studying C. lari population structure for conducting epidemiological risk assessment. This retrospective study presents a comparative genomics analysis of C. lari isolates retrieved from two different continents (Europe and Australia) and from different sources (human, domestic animals, waterbirds, food, and environment). It was designed to improve knowledge regarding C. lari ecology and pathogenicity, important for developing effective surveillance and disease prevention strategies. 
PY 2022
PD DEC
SO Applied And Environmental Microbiology
SN 0099-2240
PU American Society for Microbiology
VL 88
IS 23
UT 000883336000001
DI 10.1128/aem.01368-22
ID 91613
ER

EF