TY - JOUR T1 - Interoperable vocabulary for marine microbial flow cytometry A1 - Thyssen,Melilotus A1 - Grégori,Gérald A1 - Créach,Véronique A1 - Lahbib,Soumaya A1 - Dugenne,Mathilde A1 - Aardema,Hedy M. A1 - Artigas,Luis-Felipe A1 - Huang,Bangqin A1 - Barani,Aude A1 - Beaugeard,Laureen A1 - Bellaaj-Zouari,Amel A1 - Beran,Alfred A1 - Casotti,Raffaella A1 - Del Amo,Yolanda A1 - Denis,Michel A1 - Dubelaar,George B.J. A1 - Endres,Sonja A1 - Haraguchi,Lumi A1 - Karlson,Bengt A1 - Lambert,Christophe A1 - Louchart,Arnaud A1 - Marie,Dominique A1 - Moncoiffé,Gwenaëlle A1 - Pecqueur,David A1 - Ribalet,François A1 - Rijkeboer,Machteld A1 - Silovic,Tina A1 - Silva,Ricardo A1 - Marro,Sophie A1 - Sosik,Heidi M. A1 - Sourisseau,Marc A1 - Tarran,Glen A1 - Van Oostende,Nicolas A1 - Zhao,Li A1 - Zheng,Shan AD - Aix Marseille Université, Université de Toulon, CNRS, IRD, MIO, Marseille, France AD - Centre for Environment, Fisheries and Aquaculture, Lowestoft, United Kingdom AD - Sorbonne Université, CNRS, UMR 7093, Laboratoire d’Océanographie de Villefranche-sur-Mer (LOV), Villefranche-sur-Mer, France AD - Department of Climate Geochemistry, Max Plank Institute for Chemistry, Mainz, Germany AD - Université du Littoral Côte d’Opale, Université de Lille, IRD, CNRS, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France AD - National Observation and Research Station for the Taiwan Strait Marine Ecosystem, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China AD - Laboratoire LIENSs Littoral, Environnement et Sociétés, UMR 7266 Université de La Rochelle - CNRS, La Rochelle, France AD - Institut National des Sciences et Technologies de la Mer, INSTM, Salammbô, Tunisia AD - Physical Oceanography Group, National Institute of Oceanography and Applied Geophysics – OGS, Trieste, Italy AD - Stazione Zoologica Anton Dohrn, Department of Integrative Marine Ecology, Napoli, Italy AD - Université de Bordeaux-CNRS, UMR 5805, Environnement"s et Paléoenvironnements Océaniques et Continentaux (EPOC), Arcachon, France AD - Cytobuoy b.v., Woerden, Netherlands AD - Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Project Polarstern II, Bremerhaven, Germany AD - Marine Research Centre, Finnish Environment Institute, Helsinki, Finland AD - Swedish Meteorological and Hydrological Institute, Oceanographic Research, Västra Frölunda, Sweden AD - CNRS, Univ Brest, IRD, Ifremer, LEMAR, Plouzane, France AD - Sorbonne University, UPMC Univ Paris 06, CNRS, UMR 7144, Roscoff, France AD - National Oceanography Centre, British Oceanographic Data Centre, Liverpool, United Kingdom AD - Sorbonne Université, CNRS, Observatoire Océanologique de Banyuls s/mer, Banyuls sur Mer, France AD - School of Oceanography, University of Washington, Seattle, WA, United States AD - Laboratory for Hydrobiological Analysis, Rijkswaterstaat (RWS), Lelystad, Netherlands AD - Mercator Ocean International, Ramonville Saint - Agne, France AD - Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Buenos Aires, Argentina AD - Biology Department, Woods Hole Oceanographic Institution, Biology Department, Woods Hole, MA, United States AD - IFREMER, DYNECO, Pelagos Laboratory, Plouzané, France AD - Plymouth Marine Laboratory, Plymouth, United Kingdom AD - Department of Geosciences, Princeton University, Princeton, NJ, United States AD - Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China UR - https://archimer.ifremer.fr/doc/00806/91756/ DO - 10.3389/fmars.2022.975877 KW - flow cytometry KW - marine microorganisms KW - standardization KW - vocabulary KW - FAIR principle interoperable vocabulary for marine flow cytometry N2 - The recent development of biological sensors has extended marine plankton studies from conducting laboratory bench work to in vivo and real-time observations. Flow cytometry (FCM) has shed new light on marine microorganisms since the 1980s through its single-cell approach and robust detection of the smallest cells. FCM records valuable optical properties of light scattering and fluorescence from cells passing in a single file in front of a narrow-collimated light source, recording tens of thousands of cells within a few minutes. Depending on the instrument settings, the sampling strategy, and the automation level, it resolves the spatial and temporal distribution of microbial marine prokaryotes and eukaryotes. Cells are usually classified and grouped on cytograms by experts and are still lacking standards, reducing data sharing capacities. Therefore, the need to make FCM data sets FAIR (Findability, Accessibility, Interoperability, and Reusability of digital assets) is becoming critical. In this paper, we present a consensus vocabulary for the 13 most common marine microbial groups observed with FCM using blue and red-light excitation. The authors designed a common layout on two-dimensional log-transformed cytograms reinforced by a decision tree that facilitates the characterization of groups. The proposed vocabulary aims at standardising data analysis and definitions, to promote harmonisation and comparison of data between users and instruments. This represents a much-needed step towards FAIRification of flow cytometric data collected in various marine environments. Y1 - 2022/11 PB - Frontiers Media SA JF - Frontiers In Marine Science SN - 2296-7745 VL - 9 ID - 91756 ER -