NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of Vibrio diabolicus CNCM I-1629
|Author(s)||Drouillard Sophie1, Poulet Laurent1, Boisset Claire1, Delbarre-Ladrat Christine2, Helbert William1|
|Affiliation(s)||1 : CERMAV, CNRS and Grenoble Alpes Université, 38000 Grenoble, France
2 : Ifremer, MASAE, EM3B Laboratory, F-44300 Nantes, France
|Source||Marine Drugs (1660-3397) (mdpi), 2022-12 , Vol. 20 , N. 12 , P. 731 (8p.)|
|Note||This is an early access version, the complete PDF, HTML, and XML versions will be available soon. This article belongs to the Special Issue Poly- and Oligosaccharides from Marine Origins|
|Keyword(s)||exopolysaccharide, diabolican, hyaluronan lyase, NMR|
Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium Vibrio diabolicus (CNCM I-1629). This polysaccharide was enzymatically degraded by the Bacteroides cellulosilyticus WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in depth by nuclear magnetic resonance (NMR). The oligosaccharide structures confirmed the possible site of cleavage of the enzyme showing plasticity in the substrate recognitions. The production of glycosaminoglycan-mimetic oligosaccharides of defined molecular weight and structure opens new perspectives in the valorization of the marine polysaccharide diabolican.