FN Archimer Export Format PT J TI NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of Vibrio diabolicus CNCM I-1629 BT AF DROUILLARD, Sophie Poulet, Laurent BOISSET, Claire DELBARRE-LADRAT, Christine Helbert, William AS 1:1;2:1;3:1;4:2;5:1; FF 1:;2:;3:;4:PDG-RBE-MASAE-LEMMMB;5:; C1 CERMAV, CNRS and Grenoble Alpes Université, 38000 Grenoble, France Ifremer, MASAE, EM3B Laboratory, F-44300 Nantes, France C2 CNRS, FRANCE IFREMER, FRANCE SI NANTES SE PDG-RBE-MASAE-LEMMMB IN WOS Ifremer UPR DOAJ copubli-france IF 5.4 TC 1 UR https://archimer.ifremer.fr/doc/00806/91828/97788.pdf LA English DT Article DE ;exopolysaccharide;diabolican;hyaluronan lyase;NMR AB Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium Vibrio diabolicus (CNCM I-1629). This polysaccharide was enzymatically degraded by the Bacteroides cellulosilyticus WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in depth by nuclear magnetic resonance (NMR). The oligosaccharide structures confirmed the possible site of cleavage of the enzyme showing plasticity in the substrate recognitions. The production of glycosaminoglycan-mimetic oligosaccharides of defined molecular weight and structure opens new perspectives in the valorization of the marine polysaccharide diabolican. PY 2022 PD DEC SO Marine Drugs SN 1660-3397 PU mdpi VL 20 IS 12 UT 000900908200001 DI 10.3390/md20120731 ID 91828 ER EF