FN Archimer Export Format
PT J
TI Microbiota of the Rearing Water of Penaeus stylirostris Larvae Influenced by Lagoon Seawater and Specific Key Microbial Lineages of Larval Stage and Survival
BT 
AF Callac, Nolwenn
   Boulo, Viviane
   Giraud, Carolane
   Beauvais, Maxime
   Ansquer, Dominique
   Ballan, Valentine
   MAILLIEZ, Jean-Rene
   Wabete, Nelly
   Pham, Dominique
AS 1:1;2:1;3:1,2;4:1;5:1;6:1;7:1;8:1;9:1;
FF 1:PDG-RBE-LEADNC;2:PDG-RBE-LEADNC;3:;4:PDG-RBE-LEADNC;5:PDG-RBE-LEADNC;6:PDG-RBE-LEADNC;7:PDG-RBE-LEADNC;8:PDG-RBE-LEADNC;9:PDG-RBE-LEADNC;
C1 Ifremer, IRD, Université de la Nouvelle-Calédonie, Université de La Réunion, CNRS, UMR 9220 ENTROPIE, Nouméa, New Caledonia
   Institut des Sciences Exactes et Appliquées (ISEA), University of New Caledonia, Nouméa, New Caledonia
C2 IFREMER, FRANCE
   UNIV NOUVELLE CALEDONIE, FRANCE
SI SAINT VINCENT
SE PDG-RBE-LEADNC
UM ENTROPIE
IN WOS Ifremer UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.7
TC 5
UR https://archimer.ifremer.fr/doc/00807/91912/97866.pdf
   https://archimer.ifremer.fr/doc/00807/91912/97867.pdf
LA English
DT Article
DE ;active microbiota;rearing water;core microbiome;specific microbiome;larvae;shrimp
AB Aquacultured animals are reared in water, where they interact with microorganisms which can be involved in their development, immunity, and disease. It is therefore interesting to study the rearing water microbiota, especially in the hatcheries of the Pacific blue shrimp Penaeus stylirostris, where larval mass mortalities occur. In this study, using HiSeq sequencing of the V4 region of the 16S rRNA molecule coupled with zootechnical and chemical analyses, we investigated whether any microbial lineages could be associated with certain mortality rates at a given larval stage. Our results indicate that the active microbiota of the rearing water was highly dynamic throughout the rearing process, with distinct communities influenced by progressive water eutrophication, larval stage, and survival rate. Our data also highlighted the role of the lagoon seawater on the rearing water microbiome, as many operational taxonomic units (OTUs) specific to a given larval stage and survival rate were detected in the primary reservoir which contained the lagoon water. We also identified biomarkers specific to water eutrophication, with Alteromonadaceae, Vibrionaceae, and Methylophilaceae, respectively, linked to increases in ammonia, nitrogen, and soluble reactive phosphate, or to increases in colored dissolved organic matter in the rearing water; other biomarkers were specific to certain larval stages and survival rates. Indeed, the Marinobacteraceae were specific to the Nauplii, and the Thalassospiraceae and Saprospiraceae to the Zoea Good condition; when mortality occurred, the Litoricolaceae were specific to the Zoea Bad, Microbacteraceae to the Mysis Bad, and Methylophilaceae to the Mysis Worst condition. Thus, these biomarkers might be used as potential early warning sentinels in water storage to infer the evolution of larval rearing to improve shrimp larval rearing. IMPORTANCE In New Caledonia, rearing of P. stylirostris is one of the main economic activities; unfortunately, mass larval mortalities cause important production decreases, involving major economic losses for the farmers and the Territory. This phenomenon, which has occurred at any larval stage over the past decade, is poorly understood. The significance of our research is in the identification of biomarkers specific to larval stage and survival rate, with some of these biomarkers being already present in the lagoon water. This enhances the role of the lagoon on the active microbiota of the rearing water at various larval stages and survival rates. Together, our results help us understand which active microbial communities are present in the rearing water according to larval stage and health. This might lead to broader impacts on hatcheries by helping to develop useful tools for using the water—lagoon, reservoir, or rearing—to test for the presence of these biomarkers as an early monitoring strategy. 
PY 2022
PD DEC
SO Microbiology Spectrum
SN 2165-0497
PU American Society for Microbiology
VL 10
IS 6
UT 000889564800001
DI 10.1128/spectrum.04241-22
ID 91912
ER

EF