FN Archimer Export Format
PT J
TI Functional Diversification of Oyster Big Defensins Generates Antimicrobial Specificity and Synergy against Members of the Microbiota
BT 
AF De San Nicolas, Noémie
   Asokan, Aromal
   Rosa, Rafael D.
   Voisin, Sébastien N.
   TRAVERS, Agnes
   Rocha, Gustavo
   Dantan, Luc
   DORANT, Yann
   Mitta, Guillaume
   Petton, Bruno
   Charrière, Guillaume M.
   ESCOUBAS, Jean Michel
   Boulo, Viviane
   Pouzadoux, Juliette
   Meudal, Hervé
   Loth, Karine
   Aucagne, Vincent
   Delmas, Agnès F.
   Bulet, Philippe
   Montagnani, Caroline
   DESTOUMIEUX GARZON, Delphine
AS 1:9;2:2;3:3;4:4;5:1;6:3;7:9;8:1;9:1,5;10:6;11:9;12:10;13:9;14:9;15:2;16:2,7;17:2;18:2;19:4,8;20:1;21:10;
FF 1:;2:;3:;4:;5:PDG-RBE-IHPE;6:;7:;8:PDG-RBE-IHPE;9:PDG-RBE-RMPF;10:PDG-RBE-PFOM-LPI;11:;12:;13:PDG-RBE-IHPE;14:PDG-RBE-IHPE;15:;16:;17:;18:;19:;20:PDG-RBE-IHPE;21:;
C1 IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, 34090 Montpellier, France
   Centre de Biophysique Moléculaire UPR4301 CNRS, 45071 Orléans, France
   Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, Santa Catarina, Brazil
   Plateforme BioPark d’Archamps, Archparc, 74160 Archamps, France
   Ifremer, IRD, ILM, Université de Polynésie Française, UMR EIO, 98179 Vairao, French Polynesia
   Ifremer, CNRS, IRD, Ifremer, LEMAR UMR 6539 Université de Bretagne Occidentale, 29840 Argenton-en-Landunvez, France
   UFR ST, Université d’Orléans, 45067 Orléans, France
   CR UGA, IAB, INSERM U1209, CNRS UMR5309, 74160 La Tronche-Archamps, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, 34090 Montpellier, France
   IHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, 34090 Montpellier, France
C2 IFREMER, FRANCE
   CNRS, FRANCE
   UNIV FED SANTA CATARINA, BRAZIL
   PLATEFORME BIOPARK D’ARCHAMPS, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV ORLEANS, FRANCE
   CNRS, FRANCE
   UNIV MONTPELLIER, FRANCE
   CNRS, FRANCE
SI MONTPELLIER
   TAHITI
   ARGENTON
SE PDG-RBE-IHPE
   PDG-RBE-RMPF
   PDG-RBE-PFOM-LPI
UM LEMAR
   IHPE
   EIO
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 5.4
TC 3
UR https://archimer.ifremer.fr/doc/00807/91932/97892.pdf
   https://archimer.ifremer.fr/doc/00807/91932/97893.zip
LA English
DT Article
DE ;antibacterial peptide;beta defensin;diversity;evolution;microbiome;invertebrate;mollusk
AB Big defensins are two-domain antimicrobial peptides (AMPs) that have highly diversified in mollusks. Cg-BigDefs are expressed by immune cells in the oyster Crassostrea gigas, and their expression is dampened during the Pacific Oyster Mortality Syndrome (POMS), which evolves toward fatal bacteremia. We evaluated whether Cg-BigDefs contribute to the control of oyster-associated microbial communities. Two Cg-BigDefs that are representative of molecular diversity within the peptide family, namely Cg-BigDef1 and Cg-BigDef5, were characterized by gene cloning and synthesized by solid-phase peptide synthesis and native chemical ligation. Synthetic peptides were tested for antibacterial activity against a collection of culturable bacteria belonging to the oyster microbiota, characterized by 16S sequencing and MALDI Biotyping. We first tested the potential of Cg-BigDefs to control the oyster microbiota by injecting synthetic Cg-BigDef1 into oyster tissues and analyzing microbiota dynamics over 24 h by 16S metabarcoding. Cg-BigDef1 induced a significant shift in oyster microbiota β-diversity after 6 h and 24 h, prompting us to investigate antimicrobial activities in vitro against members of the oyster microbiota. Both Cg-BigDef1 and Cg-BigDef5 were active at a high salt concentration (400 mM NaCl) and showed broad spectra of activity against bacteria associated with C. gigas pathologies. Antimicrobial specificity was observed for both molecules at an intra- and inter-genera level. Remarkably, antimicrobial spectra of Cg-BigDef1 and Cg-BigDef5 were complementary, and peptides acted synergistically. Overall, we found that primary sequence diversification of Cg-BigDefs has generated specificity and synergy and extended the spectrum of activity of this peptide family. 
PY 2022
PD DEC
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 20
IS 12
UT 000902639900001
DI 10.3390/md20120745
ID 91932
ER

EF