FN Archimer Export Format
PT J
TI An integrated approach to determine interactive genotoxic and global gene expression effects of multiwalled carbon nanotubes (MWCNTs) and benzo[a]pyrene (BaP) on marine mussels: evidence of reverse ‘Trojan Horse’ effects
BT 
AF BARRANGER, Audrey
   RANCE, Graham A.
   AMINOT, Yann
   DALLAS, Lorna J.
   SFORZINI, Susanna
   WESTO, Nicola J.
   LODGE, Rhys W.
   BANNIE, Mohamed
   ARLT, Volker M.
   MOORE, Michael N.
   READMAND, James W.
   VIARENGO, Aldo
   KHLOBYSTOV, Andrei N.
   JHA, Awadhesh N.
AS 1:1,12;2:2,3;3:4;4:1;5:5;6:3;7:2,3;8:5,6;9:7,8,9;10:1,10,11;11:4,10;12:5;13:2,3;14:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;
C1 Univ Plymouth, Sch Biol & Marine Sci, Plymouth PL4 8AA, Devon, England.
   Univ Nottingham, Sch Chem, Univ Park, Nottingham, England.
   Univ Nottingham, Nanoscale & Microscale Res Ctr, Nottingham, England.
   Univ Plymouth, Ctr Chem Sci, Plymouth, Devon, England.
   Ist Ric Farmacol Mario Negri IRCCS, Ecotoxicol & Environm Safety Unit, Milan, Italy.
   Sousse Univ, ISA ChottMariem, Lab Biochem & Environm Toxicol, Sousse, Tunisia.
   Kings Coll London, MRC, PHE Ctr Environm & Hlth, Dept Analyt Environm & Forens Sci, London, England.
   Kings Coll London, Partnership Publ Hlth England, Hlth Impact Environm Hazards, NIHR Hlth Protect Res Unit, London, England.
   Imperial Coll London, London, England.
   Plymouth Marine Lab, Plymouth, Devon, England.
   Univ Exeter, Med Sch, Royal Cornwall Hosp, ECEHH, Truro, England.
   Univ Rennes, Ctr Natl Rech Sci 1, UMR ECOBIO 6553, F-35000 Rennes, France.
C2 UNIV PLYMOUTH, UK
   UNIV NOTTINGHAM, UK
   UNIV NOTTINGHAM, UK
   UNIV PLYMOUTH, UK
   IRCCS, ITALY
   UNIV SOUSSE, TUNISIA
   KINGS COLL LONDON, UK
   KINGS COLL LONDON, UK
   IMPERIAL COLL LONDON, UK
   PML, UK
   UNIV EXETER, UK
   UNIV RENNES, FRANCE
IF 4.925
TC 10
UR https://archimer.ifremer.fr/doc/00809/92103/98054.pdf
LA English
DT Article
DE ;Mytilus galloprovincialis;benzo[a]pyrene;multi-walled carbon nanotubes;van der Waals interactions;adsorption
AB The interactions between carbon-based engineered nanoparticles (ENPs) and organic pollutants might enhance the uptake of contaminants into biota. The present integrated study aimed to assess this potential 'Trojan Horse', probing the interactive effects of purpose-made multi-walled carbon nanotubes (MWCNTs), a representative ENP, and benzo[a]pyrene (BaP), a ubiquitous polycyclic aromatic hydrocarbon (PAH) pollutant, on the marine mussel Mytilus galloprovincialis. Mussels were exposed to MWCNTs and BaP either alone or in various combinations. The co-exposure of BaP with MWCNTs revealed that the presence of MWCNTs enhanced the aqueous concentrations of BaP, thereby reducing the uptake of this pollutant by mussels as evidenced by lowering BaP concentrations in the tissues. Determination of DNA damage (comet assay) showed a concentration-dependent response for BaP alone which was absent when MWCNTs were present. Global gene expression using microarray analyses indicated that BaP and MWCNTs, in combination, differentially activated those genes which are involved in DNA metabolism compared to the exposures of BaP or MWCNTs alone, and the gene expression response was tissue-specific. Mechanisms to explain these results are discussed and relate primarily to the adsorption of BaP on MWCNTs, mediated potentially by van der Waals interactions. The use of a novel approach based on gold-labeled MWCNTs to track their uptake in tissues improved the traceability of nanotubes in biological samples. Overall, our results did not indicate the 'Trojan Horse' effects following co-exposure to the contaminants and clearly showed that the adsorption of BaP to MWCNTs modified the uptake of the pollutant in marine mussels. 
PY 2019
PD NOV
SO Nanotoxicology
SN 1743-5390
PU Taylor & Francis Ltd
VL 13
IS 10
UT 000484636700001
BP 1324
EP 1343
DI 10.1080/17435390.2019.1654003
ID 92103
ER

EF