FN Archimer Export Format
PT J
TI m6A Profile Dynamics Indicates Regulation of Oyster Development by m6A-RNA Epitranscriptomes
BT 
AF Le Franc, Lorane
   Petton, Bruno
   Favrel, Pascal
   Rivière, Guillaume
AS 1:1;2:2;3:1;4:1;
FF 1:;2:PDG-RBE-PFOM-LPI;3:;4:;
C1 Laboratoire de Biologie des Organismes et des Ecosystèmes Aquatiques (BOREA), Muséum d’Histoire Naturelle, Sorbonne Université, Université de Caen Normandie, Université des Antilles, CNRS UMR 8067, IRD, 14032 Caen, France
   Ifremer, Laboratoire des Sciences de l’Environnement Marin, UMR 6539 CNRS/UBO/IRD/Ifremer, Centre Bretagne, 29280 Plouzané, France
C2 MNHN, FRANCE
   IFREMER, FRANCE
SI ARGENTON
SE PDG-RBE-PFOM-LPI
UM LEMAR
IN WOS Ifremer UMR
   DOAJ
   copubli-france
IF 9.5
TC 1
UR https://archimer.ifremer.fr/doc/00809/92110/98082.pdf
   https://archimer.ifremer.fr/doc/00809/92110/107259.pptx
   https://archimer.ifremer.fr/doc/00809/92110/107260.pptx
   https://archimer.ifremer.fr/doc/00809/92110/107261.xls
LA English
DT Article
DE ;RNA methylation;Transcription;Embryo;Metamorphosis;Evo-devo
AB The N6-methylation of RNA adenosines (N6-methyladenosine, m6A) is an important regulator of gene expression with critical implications in vertebrate and insect development. However, the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown. Using methylated RNA immunoprecipitation sequencing (MeRIP-seq), we generated transcriptome-wide m6A-RNA methylomes covering the whole development of the oyster from oocytes to juveniles. Oyster RNA classes display specific m6A signatures, with messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) exhibiting distinct profiles and being highly methylated compared to transposon transcripts. Epitranscriptomes are dynamic and correspond to the chronological steps of development (cleavage, gastrulation, organogenesis, and metamorphosis), with a minimal mRNA and lncRNA methylation at the morula stage followed by a global increase. mRNA m6A levels are correlated to transcript levels and shifts in methylation profiles correspond to expression kinetics. Differentially methylated transcripts cluster according to embryo-larval stages and bear the corresponding developmental functions (cell division, signal transduction, morphogenesis, and cell differentiation). The m6A level of transposon transcripts is also regulated and peaks during the gastrulation. We demonstrate that m6A-RNA methylomes are dynamic and associated to gene expression regulation during oyster development. The putative epitranscriptome implication in the cleavage, maternal-to-zygotic transition, and cell differentiation in a lophotrochozoan model brings new insights into the control and evolution of developmental processes. 
PY 2023
PD AUG
SO Genomics Proteomics & Bioinformatics
SN 1672-0229
PU Elsevier BV
VL 21
IS 4
UT 001155907900001
BP 742
EP 755
DI 10.1016/j.gpb.2022.12.002
ID 92110
ER

EF