FN Archimer Export Format
PT J
TI A novel pectic polysaccharide-based hydrogel derived from okra (Abelmoschus esculentus L. Moench) for chronic diabetic wound healing
BT 
AF Maalej, Hana
   Maalej, Amina
   Bayach, Asma
   Zykwinska, Agata
   COLLIEC JOUAULT, Sylvia
   Sinquin, Corinne
   Marchand, Laetitia
   Ktari, Naourez
   Bardaa, Sana
   Ben Salah, Riadh
   Chamkha, Mohamed
   Boufi, Sami
   Nasri, Moncef
AS 1:1;2:2;3:1;4:3;5:3;6:3;7:3;8:4;9:5;10:6;11:2;12:7;13:8;
FF 1:;2:;3:;4:PDG-RBE-MASAE-LEMMMB;5:PDG-RBE-MASAE-LEMMMB;6:PDG-RBE-MASAE-LEMMMB;7:PDG-RBE-BRM-LEMMMB;8:;9:;10:;11:;12:;13:;
C1 University of Gabes, Faculty of Sciences of Gabes, Laboratory of Biodiversity and Valorization of Arid Areas Bioresources (BVBAA), LR16ES36, Faculty of Sciences, Erriadh 6072, Gabes, Tunisia
   Environmental Bioprocesses Laboratory, Sfax Biotechnology Center, P.O. Box 1177, Sfax 3018, Tunisia
   Ifremer, MASAE, 44300 Nantes, France
   University of Gabes, Faculty of Sciences of Gabes, Department of Life Sciences, Gabes 6072, Tunisia
   Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Tunisia
   Laboratory of Microorganisms and Biomolecules, Sfax Biotechnology Center, 3018 Sfax, Tunisia
   University of Sfax- LMSE-Faculty of Science-BP 802-3018, Sfax, Tunisia
   Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax 3038, Tunisia
C2 UNIV GABES, TUNISIA
   UNIV SFAX, TUNISIA
   IFREMER, FRANCE
   UNIV GABES, TUNISIA
   UNIV SFAX, TUNISIA
   UNIV SFAX, TUNISIA
   UNIV SFAX, TUNISIA
   UNIV SFAX, TUNISIA
SI NANTES
SE PDG-RBE-MASAE-LEMMMB
   PDG-RBE-BRM-LEMMMB
IN WOS Ifremer UPR
   copubli-int-hors-europe
   copubli-sud
IF 6
TC 6
UR https://archimer.ifremer.fr/doc/00811/92292/98339.pdf
LA English
DT Article
DE ;Okra polysaccharide;Pectin;Physicochemical features;Rheological properties;Antioxidant potential;Hydrogel;Wound healing;Scratch assay;Cell migration;Diabetic wound
AB Hydrogels based on natural polysaccharides represent a growing group of suitable biomaterials for the elaboration of effective wound healing dressings, especially for the treatment of chronic wounds. This work was intended to prepare a polysaccharide-based hydrogel for diabetic wound healing which would help maintain the well-being of diabetes and improve their quality of life. For this purpose, a pectic polysaccharide (OPS) was extracted and purified, for the first time, from Tunisian okra pods and its physicochemical and rheological features, antioxidant and in vivo and in vitro wound healing activities were investigated. OPS, an acidic polysaccharide with a molecular weight of 3.28 × 106 Da and a polydispersity index of 1.03, was mainly composed of galactose (24.45%), galacturonic acid (24.6%) and rhamnose (18.25%). Combined with FT-IR and NMR analyses, it consisted of a pectic rhamnogalacturonan I (RG-I) structure with galactan side chains. The OPS demonstrated antioxidant potential, gelling ability, cytocompatibility properties, non-cytotoxicity and cell migration and proliferation promoting activities, which met the requirements for wound dressings. Then, the in vivo cutaneous wound healing effect of OPS-based hydrogel was investigated using an alloxan-induced diabetic rat model, and results showed that it significantly accelerated the wound healing process by acting in the acceleration of the recovery of the dermis and inducing more blood vessels formation and tissue granulation. Overall, these results provide new insights into the development of a promising and effective okra pectin-based hydrogel for the treatment of chronic diabetic wounds. 
PY 2023
PD JAN
SO European Polymer Journal
SN 0014-3057
PU Elsevier BV
VL 183
UT 000906803800001
DI 10.1016/j.eurpolymj.2022.111763
ID 92292
ER

EF