FN Archimer Export Format
PT J
TI Insights into the molecular mechanisms of pesticide tolerance in the Aporrectodea caliginosa earthworm
BT 
AF Barranger, Audrey
   Klopp, Christophe
   Le Bot, Barbara
   Saramito, Gaëlle
   Dupont, Lise
   Llopis, Stéphanie
   Wiegand, Claudia
   Binet, Françoise
AS 1:1;2:2;3:3;4:3;5:4;6:1;7:1;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 UMR CNRS ECOBIO 6553, Université de Rennes1, Campus de Beaulieu, 263 Avenue du Général Leclerc, 35042, Rennes, Cedex, France
   UR INRAE 875 MIAT, GENOTOUL, 24 Chemin de Borde Rouge, 31326, Castanet-Tolosan, Cedex, France
   Université de Rennes, EHESP, Inserm, Irset (Institut de Recherche en Santé, Environnement et Travail) - UMR_S 1085, F 35000, Rennes, France
   Université Paris Est Créteil (UPEC), Sorbonne Université, CNRS, INRAE, IRD, Institut d'Ecologie et des Sciences de l'Environnement de Paris, 94010, Créteil, Cedex, France
C2 UNIV RENNES, FRANCE
   INRAE, FRANCE
   UNIV RENNES, FRANCE
   UNIV PARIS EST, FRANCE
IF 8.9
TC 2
UR https://archimer.ifremer.fr/doc/00813/92484/99074.pdf
LA English
DT Article
DE ;Aporrectodea;Transcriptomics;Pesticides residues;Mitochondrial respiratory chain;Agrosystem;Soil biodiversity
AB Diffuse pollution of the environment by pesticides has become a major soil threat to non-target organisms, such as earthworms for which declines have been reported. However some endogeic species are still abundant and persist in intensively cultivated fields, suggesting they become tolerant to long-term anthropogenic pressure. We thus considered the working hypothesis that populations of Aporrectodea caliginosa earthworms from conventionally managed fields developed a tolerance to pesticides compared with those from organically managed fields. To investigate this hypothesis, we studied earthworm populations of the same genetic lineage from soils that were either lowly or highly contaminated by pesticides to detect any constitutive expression of differentially expressed molecular pathways between these populations. Earthworm populations were then experimentally exposed to a fungicide—epoxiconazole—in the laboratory to identify different molecular responses when newly exposed to a pesticide. State-of-the-art omics technology (RNA sequencing) and bioinformatics were used to characterize molecular mechanisms of tolerance in a non-targeted way. Additional physiological traits (respirometry, growth, bioaccumulation) were monitored to assess tolerance at higher levels of biological organization. In the present study, we generated the de novo assembly transcriptome of A. caliginosa consisting of 64,556 contigs with N50 = 2862 pb. In total, 43,569 Gene Ontology terms were identified for 21,593 annotated sequences under the three main ontologies (biological processes, cellular components and molecular functions). Overall, we revealed that two same lineage populations of A. caliginosa earthworms, inhabiting similar pedo-climatic environment, have distinct gene expression pathways after they long-lived in differently managed agricultural soils with a contrasted pesticide exposure history for more than 22 years. The main difference was observed regarding metabolism, with upregulated pathways linked to proteolytic activities and the mitochondrial respiratory chain in the highly exposed population. This study improves our understanding of the long-term impact of chronic exposure of soil engineers to pesticide residues. 
PY 2023
PD FEB
SO Environmental Pollution
SN 0269-7491
PU Elsevier BV
VL 319
UT 000923289700001
DI 10.1016/j.envpol.2022.120945
ID 92484
ER

EF