FN Archimer Export Format
PT J
TI Short-term effects of estradiol and bisphenol A on gene expression associated with early head mineralization in the seabass Dicentrarchus labrax
BT 
AF Farcy, Emilie
   Potier, Eric
   Leurs, Nicolas
   Gasset, Eric
   Dutto, Gilbert
   Lallement, Stephane
   Bourdy, Clarence
   Debiais-Thibaud, Mélanie
   Martinand-Mari, Camille
AS 1:3;2:3;3:2;4:1;5:1;6:1;7:3;8:2;9:2;
FF 1:;2:;3:;4:PDG-RBE-MARBEC-LAAAS;5:PDG-RBE-MARBEC-LSEA;6:PDG-RBE-MARBEC-LSEA;7:;8:;9:;
C1 Marine Biodiversity, Exploitation and Conservation, MARBEC, Univ. Montpellier, Ifremer, CNRS, IRD, Montpellier, France
   Institut des Sciences de l’Evolution de Montpellier, ISEM, Univ Montpellier, CNRS, IRD, Montpellier, France
   Marine Biodiversity, Exploitation and Conservation, MARBEC, Univ. Montpellier, Ifremer, CNRS, IRD, Montpellier, France
C2 IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   UNIV MONTPELLIER, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
   PDG-RBE-MARBEC-LSEA
UM MARBEC
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.7
TC 0
UR https://archimer.ifremer.fr/doc/00813/92501/98743.pdf
   https://archimer.ifremer.fr/doc/00813/92501/98744.zip
LA English
DT Article
DE ;estradiol;bisphenol A;skeletogenesis;bone mineralization;estrogen signaling;seabass Dicentrarchus labrax
AB IntroductionNatural and synthetic estrogens are pollutants found in aquatic ecosystems at low concentrations reaching ng.L-1 to μg.L-1. At these concentrations, they are able to interfere with the fish endocrine system. When waterborne exposure occurs at early life stages, when blood estrogens concentrations are low, this may have significant consequences for estrogen-sensitive functions such as skeletal development. MethodsTo better understand how (xeno)estrogens may affect early head mineralization, 12 days post-hatch larvae of the European seabass Dicentrarchus labrax were experimentally exposed for 4 days to the natural estrogen estradiol E2 and to the xenoestrogen bisphenol A (BPA), both used at either regulatory concentration of water quality or a 100 times higher concentration. Head mineralization level was assessed using Alizarin red staining, together with the relative quantification of mRNA expression levels of several genes playing key roles in skeletogenesis and estrogen signaling pathways. ResultsWe showed that (xeno)estrogen exposure at early larval stage increases the expression of skeleton-associated genes: matrix proteins encoding genes (col1a2, col2a1a, col2a1b, bgp1a, bgp1b, sparc), proteolytic enzyme encoding genes (ctsk) and transcription and signaling factors (sox9a, sox9b, ihha, runx2, rankl). Although transcriptional overexpression of these genes was significant in larvae exposed to 40 ng.L-1 E2 and to 1.6 and 160 μg.L-1 BPA, increased mineralization was detected only in E2-exposed larvae, suggesting a difference in head skeleton development and remodeling in BPA-treated larvae. DiscussionOur results suggest that these phenotypic differences could be due to the implication of other estrogenic signaling pathways involving both nuclear and membrane-bound estrogen receptors (ERs and GPERs), but also estrogen-related receptors (ERRs). This study brings new insights into the regulatory mechanisms of skeletogenesis by E2 and BPA and into the effects of waterborne exposure to (xeno)estrogens on the early skeletal development of teleost fishes. 
PY 2022
PD DEC
SO Frontiers In Marine Science
SN 2296-7745
PU Frontiers Media SA
VL 9
UT 000905586000001
DI 10.3389/fmars.2022.1062334
ID 92501
ER

EF