FN Archimer Export Format
PT J
TI A device for assessing microbial activity under ambient hydrostatic pressure: The in situ microbial incubator ( ISMI )
BT 
AF Amano, Chie
   Reinthaler, Thomas
   Sintes, Eva
   Varela, Marta M.
   Stefanschitz, Julia
   Kaneko, Sho
   Nakano, Yoshiyuki
   Borchert, Wolfgang
   Herndl, Gerhard J.
   Utsumi, Motoo
AS 1:1;2:1;3:2;4:3;5:1;6:4;7:5;8:6;9:1,7;10:8,9;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;
C1 Department of Functional and Evolutionary Ecology, Bio‐Oceanography Unit University of Vienna Vienna ,Austria
   Instituto Español de Oceanografía‐CSIC, Centro Oceanográfico de Baleares Palma de Mallorca, Spain
   Instituto Español de Oceanografia‐CSIC, Centro Oceanografico de A Coruña A Coruña, Spain
   NiGK Corporation Saitama ,Japan
   Japan Agency for Marine‐Earth Science and Technology (JAMSTEC) Yokosuka ,Japan
   Briese Schiffahrts GmbH & Co. KG Leer ,Germany
   NIOZ, Department of Marine Microbiology and Biogeochemistry Royal Netherlands Institute for Sea Research, Utrecht University Texel ,The Netherlands
   Faculty of Life and Environmental Sciences University of Tsukuba Ibaraki ,Japan
   Microbiology Research Center for Sustainability University of Tsukuba Ibaraki, Japan
C2 UNIV VIENNA, AUSTRIA
   IEO CSIC, SPAIN
   IEO CSIC, SPAIN
   NIGK CORPORATION, JAPAN
   JAMSTEC, JAPAN
   BRIESE SCHIFFAHRTS GMBH & CO., GERMANY
   INST SEA RESEARCH (NIOZ), NETHERLANDS
   UNIV TSUKUBA, JAPAN
   UNIV TSUKUBA, JAPAN
IF 2.7
TC 0
UR https://archimer.ifremer.fr/doc/00813/92543/98825.pdf
   https://archimer.ifremer.fr/doc/00813/92543/98826.tif
   https://archimer.ifremer.fr/doc/00813/92543/98827.dxf
   https://archimer.ifremer.fr/doc/00813/92543/98828.dxf
   https://archimer.ifremer.fr/doc/00813/92543/98829.pdf
   https://archimer.ifremer.fr/doc/00813/92543/98830.tif
   https://archimer.ifremer.fr/doc/00813/92543/98831.pdf
   https://archimer.ifremer.fr/doc/00813/92543/98832.docx
   https://archimer.ifremer.fr/doc/00813/92543/98833.docx
   https://archimer.ifremer.fr/doc/00813/92543/98834.docx
LA English
DT Article
AB Microbes in the dark ocean are exposed to hydrostatic pressure increasing with depth. Activity rate measurements and biomass production of dark ocean microbes are, however, almost exclusively performed under atmospheric pressure conditions due to technical constraints of sampling equipment maintaining in situ pressure conditions. To evaluate the microbial activity under in situ hydrostatic pressure, we designed and thoroughly tested an in situ microbial incubator (ISMI). The ISMI allows autonomously collecting and incubating seawater at depth, injection of substrate and fixation of the samples after a preprogramed incubation time. The performance of the ISMI was tested in a high-pressure tank and in several field campaigns under ambient hydrostatic pressure by measuring prokaryotic bulk 3H-leucine incorporation rates. Overall, prokaryotic leucine incorporation rates were lower at in situ pressure conditions than under to depressurized conditions reaching only about 50% of the heterotrophic microbial activity measured under depressurized conditions in bathypelagic waters in the North Atlantic Ocean off the northwestern Iberian Peninsula. Our results show that the ISMI is a valuable tool to reliably determine the metabolic activity of deep-sea microbes at in situ hydrostatic pressure conditions. Hence, we advocate that deep-sea biogeochemical and microbial rate measurements should be performed under in situ pressure conditions to obtain a more realistic view on deep-sea biotic processes. 
PY 2023
PD FEB
SO Limnology And Oceanography-methods
SN 1541-5856
PU Wiley
VL 21
IS 2
UT 000898139300001
BP 69
EP 81
DI 10.1002/lom3.10528
ID 92543
ER

EF