FN Archimer Export Format PT J TI Temperature-dependent metabolic consequences of food deprivation in the European sardine BT AF Thoral, Elisa Roussel, Damien Gasset, Eric Dutto, Gilbert Queiros, Quentin McKenzie, David J. Bourdeix, Jean-Herve Metral, Luisa Saraux, Claire Teulier, Loïc AS 1:1;2:1;3:2;4:2;5:3,4;6:5;7:3;8:3;9:3,6;10:1; FF 1:;2:;3:PDG-RBE-MARBEC-LAAAS;4:PDG-RBE-MARBEC-LSEA;5:;6:;7:PDG-RBE-MARBEC-LHM;8:PDG-RBE-MARBEC-LHM;9:PDG-RBE-MARBEC-LHM;10:; C1 Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR 5023 LEHNA, F-69622, Villeurbanne, France MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, chemin de Maguelonne, 34250 Palavas-les-Flots, France MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Avenue Jean Monnet, 34203 Sète Cedex, France DECOD (Ecosystem Dynamics and Sustainability), INRAE, Institut Agro, IFREMER, Rennes, France MARBEC, Université de Montpellier, CNRS, Ifremer, IRD, Place Eugène Bataillon, 34095 Montpellier, France IPHC, UMR7178, Université de Strasbourg, CNRS, 67000 Strasbourg, France C2 UNIV LYON, FRANCE IFREMER, FRANCE IFREMER, FRANCE INRAE, FRANCE UNIV MONTPELLIER, FRANCE UNIV STRASBOURG, FRANCE SI PALAVAS SETE SE PDG-RBE-MARBEC-LAAAS PDG-RBE-MARBEC-LSEA PDG-RBE-MARBEC-LHM UM MARBEC DECOD IN WOS Ifremer UMR WOS Cotutelle UMR copubli-france copubli-univ-france IF 2.8 TC 6 UR https://archimer.ifremer.fr/doc/00815/92664/98960.pdf LA English DT Article DE ;Bioenergetics;Red muscle;Mitochondria;Respirometry;Global warming;Small pelagic fish AB Aquatic ecosystems can exhibit seasonal variation in resource availability and animals have evolved to cope with the associated caloric restriction. During winter in the NW Mediterranean Sea, the European sardine Sardina pilchardus naturally experiences caloric restriction due to a decrease in diversity and quantity of plankton. Ongoing global warming has, however, had deleterious effects on plankton communities such that food shortages may occur throughout the year, especially under warm conditions in the summer. We investigated the interactive effects of temperature and food availability on sardine metabolism, by continuously monitoring whole-animal respiration of groups of control (fed) and food-deprived sardines over a 60-day experiment in winter (12°C) or summer (20°C) conditions under natural photoperiod. In addition, we measured mitochondrial respiration of red muscle fibres, biometric variables and energy reserves, of individuals sampled at 30 and 60 days. This revealed that winter food deprivation elicits energy saving mechanisms at whole animal and cellular levels by maintaining a low metabolism to preserve energy reserves, allowing high survival. By contrast, despite energy saving mechanisms at the mitochondrial level, whole animal metabolic rate was high during food deprivation in summer, causing increased consumption of energy reserves at the muscular level and high mortality after 60 days. Furthermore, a 5-day refeeding did not improve survival and mortalities actually continued, suggesting that long-term food deprivation at high temperatures caused profound stress in sardines that potentially impaired nutrient absorption. PY 2023 PD JAN SO Journal Of Experimental Biology SN 0022-0949 PU The Company of Biologists VL 226 IS 2 UT 001132046000007 DI 10.1242/jeb.244984 ID 92664 ER EF