FN Archimer Export Format
PT J
TI Biodegradation of 2-ethylhexyl nitrate by Mycobacterium austroafricanum IFP 2173
BT 
AF NICOLAU, Elodie
   KERHOAS, Lucien
   LETTERE, Martine
   JOUANNEAU, Yves
   MARCHAL, Remy
AS 1:1;2:2;3:2;4:3;5:1;
FF 1:;2:;3:;4:;5:;
C1 IFP, Departement de Biotechnologie et Chimie de la Biomasse, 1-4 avenue du Bois Preau, 92852 Rueil-Malmaison Cedex, France
   INRA, Unite de Phytopharmacie et Me´diateurs Chimiques, Route de St-Cyr, 78026 Versailles Cedex, France
   iRTSV, Laboratoire de Chimie et Biologie des Metaux, 17 rue des Martyrs, F-38054 Grenoble, CNRS, UMR5249, F-38054 Grenoble, and Universite Joseph Fourrier, F-38000 Grenoble, France
C2 IFP, FRANCE
   INRA, FRANCE
   UNIV GRENOBLE, FRANCE
IF 3.801
TC 6
UR https://archimer.ifremer.fr/doc/00816/92748/99054.pdf
LA English
DT Article
AB 2-Ethyhexyl nitrate (2-EHN) is a major additive of fuel that is used to increase the cetane number of diesel. Because of its wide use and possible accidental release, 2-EHN is a potential pollutant of the environment. In this study, Mycobacterium austroafricanum IFP 2173 was selected from among several strains as the best 2-EHN degrader. The 2-EHN biodegradation rate was increased in biphasic cultures where the hydrocarbon was dissolved in an inert non-aqueous-phase liquid, suggesting that the transfer of the hydrophobic substrate to the cells was a growth-limiting factor. Carbon balance calculation, as well as organic-carbon measurement, indicated a release of metabolites in the culture medium. Further analysis by gas chromatography revealed that a single metabolite accumulated during growth. This metabolite had a molecular mass of 114 Da as determined by gas chromatography/mass spectrometry and was provisionally identified as 4-ethyldihydrofuran2(3H)-one by liquid chromatography-tandem mass spectrometry analysis. Identification was confirmed by analysis of the chemically synthesized lactone. Based on these results, a plausible catabolic pathway is proposed whereby 2-EHN is converted to 4-ethyldihydrofuran-2(3H)-one, which cannot be metabolized further by strain IFP 2173. This putative pathway provides an explanation for the low energetic efficiency of 2-EHN degradation and its poor biodegradability. 
PY 2008
PD OCT
SO Applied And Environmental Microbiology
SN 0099-2240
PU Amer Soc Microbiology
VL 74
IS 20
UT 000259985300003
BP 6187
EP 6193
DI 10.1128/AEM.01142-08
ID 92748
ER

EF