FN Archimer Export Format PT J TI Total mineralization of 2-ethylhexyl nitrate by bacterial cocultures BT AF NICOLAU, Elodie DELLA GIUSTINA, Gabriela JOUANNEAU, Yves MARCHAL, Remy AS 1:1,2;2:1;3:2;4:1; FF 1:;2:;3:;4:; C1 Department of Biotechnology, Institut Français du Pétrole (IFP), 1-4 Avenue de Bois Préau, 92852, Rueil-Malmaison, France CEA, IRTSV, LCBM, Grenoble, France C2 IFP, FRANCE CEA, FRANCE IF 1.214 TC 0 UR https://archimer.ifremer.fr/doc/00816/92750/99296.pdf LA English DT Article DE ;2-EHN;Biodegradability;Cocultures;Cetane index AB 2-Ethylhexyl nitrate (2-EHN) is a widely-used chemical which is commonly added to diesel oil to boost its cetane index. The 2-EHN molecule is recalcitrant to biodegradation but still utilized as sole carbon source by Mycobacterium austroafricanum IFP 2173. The incomplete degradation of 2-EHN by this strain results in the accumulation of an intermediary metabolite i.e. 4-ethyldihydrofuran-2(3H)-one (4-EDF). The study aimed at isolating 4-EDF degraders in order to achieve total mineralization of 2-EHN in cocultures with M. austroafricanum IFP 2173. Bacterial isolates were obtained from diesel-contaminated soil by enrichment in serial cultures supplemented with 4-EDF, the degradation of which was monitored by CO2 measurements. Two strains were isolated and identified as Bacillus cereus and Burkholderia sp., respectively. Complete mineralization of 2-EHN was achieved by associating M. austroafricanum IFP 2173 with either bacterial isolate in cocultures. In the context of environmental acceptability, efficient degradation of a potentially persistent pollutant by a bacterial consortium is demonstrated. PY 2010 PD NOV SO World Journal Of Microbiology & Biotechnology SN 0959-3993 PU Springer VL 26 IS 11 UT 000282507400023 BP 2113 EP 2116 DI 10.1007/s11274-010-0395-6 ID 92750 ER EF