FN Archimer Export Format
PT J
TI DNA Polymerization in Icy Moon Abyssal Pressure Conditions
BT 
AF Carré, Lorenzo
   Henneke, Ghislaine
   Henry, Etienne
   Flament, Didier
   Girard, Éric
   Franzetti, Bruno
AS 1:1;2:2;3:3;4:2;5:1;6:1;
FF 1:;2:PDG-REM-BEEP-LMEE;3:;4:PDG-REM-BEEP-LMEE;5:;6:;
C1 University of Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France.
   Laboratoire de Microbiologie des Environnements Extrêmes, CNRS, Ifremer, Université de Brest, Plouzané, France.
   Laboratoire de Microbiologie des Environnements Extrêmes, CNRS, Ifremer, Université de Brest, Plouzané, France.
C2 UNIV GRENOBLE ALPES, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
SI BREST
SE PDG-REM-BEEP-LMEE
UM BEEP-LM2E
IF 4.2
TC 0
UR https://archimer.ifremer.fr/doc/00816/92758/99155.pdf
LA English
DT Article
AB Evidence of stable liquid water oceans beneath the ice crust of moons within the Solar System is of great interest for astrobiology. In particular, subglacial oceans may present hydrothermal processes in their abysses, similarly to terrestrial hydrothermal vents. Therefore, terrestrial extremophilic deep life can be considered a model for putative icy moon extraterrestrial life. However, the comparison between putative extraterrestrial abysses and their terrestrial counterparts suffers from a potentially determinant difference. Indeed, some icy moons oceans may be so deep that the hydrostatic pressure would exceed the maximal pressure at which hydrothermal vent organisms have been isolated. While terrestrial microorganisms that are able to survive in such conditions are known, the effect of high pressure on fundamental biochemical processes is still unclear. In this study, the effects of high hydrostatic pressure on DNA synthesis catalyzed by DNA polymerases are investigated for the first time. The effect on both strand displacement and primer extension activities is measured, and pressure tolerance is compared between enzymes of various thermophilic organisms isolated at different depths. 
PY 2024
PD FEB
SO Astrobiology
SN 1531-1074
PU Mary Ann Liebert Inc
VL 24
IS 2
BP 151
EP 162
DI 10.1089/ast.2021.0201
ID 92758
ER

EF