FN Archimer Export Format
PT J
TI An aphid symbiont confers protection against a specialized RNA virus, another increases vulnerability to the same pathogen
BT 
AF Higashi, Clesson H. V.
   Nichols, William L.
   Chevignon, Germain
   Patel, Vilas
   Allison, Suzanne E.
   Kim, Kyungsun Lee
   Strand, Michael R.
   Oliver, Kerry M.
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:1;8:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;
C1 Department of Entomology, University of Georgia Georgia Athens ,USA
C2 UNIV GEORGIA, USA
IF 4.9
TC 7
UR https://archimer.ifremer.fr/doc/00816/92847/99209.pdf
   https://archimer.ifremer.fr/doc/00816/92847/99210.pdf
   https://archimer.ifremer.fr/doc/00816/92847/99211.pdf
   https://archimer.ifremer.fr/doc/00816/92847/99213.pdf
LA English
DT Article
DE ;Acyrthosiphon pisum virus;defensive symbiosis;endosymbiont;Hamiltonella defensa;heritable symbiont;pea aphid;Regiella insecticola;symbiont
AB Insects often harbour heritable symbionts that provide defence against specialized natural enemies, yet little is known about symbiont protection when hosts face simultaneous threats. In pea aphids (Acyrthosiphon pisum), the facultative endosymbiont Hamiltonella defensa confers protection against the parasitoid, Aphidius ervi, and Regiella insecticola protects against aphid-specific fungal pathogens, including Pandora neoaphidis. Here, we investigated whether these two common aphid symbionts protect against a specialized virus A. pisum virus (APV), and whether their antifungal and antiparasitoid services are impacted by APV infection. We found that APV imposed large fitness costs on symbiont-free aphids and these costs were elevated in aphids also housing H. defensa. In contrast, APV titres were significantly reduced and costs to APV infection were largely eliminated in aphids with R. insecticola. To our knowledge, R. insecticola is the first aphid symbiont shown to protect against a viral pathogen, and only the second arthropod symbiont reported to do so. In contrast, APV infection did not impact the protective services of either R. insecticola or H. defensa. To better understand APV biology, we produced five genomes and examined transmission routes. We found that moderate rates of vertical transmission, combined with horizontal transfer through food plants, were the major route of APV spread, although lateral transfer by parasitoids also occurred. Transmission was unaffected by facultative symbionts. In summary, the presence and species identity of facultative symbionts resulted in highly divergent outcomes for aphids infected with APV, while not impacting defensive services that target other enemies. These findings add to the diverse phenotypes conferred by aphid symbionts, and to the growing body of work highlighting extensive variation in symbiont-mediated interactions. 
PY 2023
PD FEB
SO Molecular Ecology
SN 0962-1083
PU Wiley
VL 32
IS 4
UT 000900004700001
BP 936
EP 950
DI 10.1111/mec.16801
ID 92847
ER

EF