FN Archimer Export Format
PT J
TI Methanocaldococcus lauensis sp. nov., a novel deep-sea hydrothermal vent hyperthermophilic methanogen
BT 
AF L'Haridon, Stephane
   Goulaouic, Steven
   St John, Emily
   Fouteau, Stephanie
   Reysenbach, Anna-Louise
AS 1:1;2:1;3:2;4:3;5:2;
FF 1:;2:;3:;4:;5:;
C1 Univ Brest, CNRS, IFREMER, Unité Biologie et Ecologie des Ecosystèmes Marins Profonds, F-29280, Plouzané, France
   Department of Biology and Center for Life in Extreme Environments, Portland State University, P.O.Box 751 Portland, OR 97207, USA
   Génomique Métabolique, CEA, Genoscope, Institut François Jacob, Université d’Évry and Université Paris-Saclay, CNRS, Evry, France
C2 UBO, FRANCE
   UNIV PORTLAND STATE, USA
   UNIV PARIS SACLAY, FRANCE
UM BEEP-LM2E
IN WOS Cotutelle UMR
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 2.8
TC 0
UR https://archimer.ifremer.fr/doc/00818/92988/99637.pdf
LA English
DT Article
CR CHUBACARC
BO L'Atalante
DE ;deep-sea hydrothermal vent;Euryarchaeota;Methanocaldococcaceae;Methanococcales;Methanococci;Methanocaldococcus
AB Three hyperthermophilic methanogens, designated strain SG7T, strain SG1 and strain SLH, were isolated from the ABE and Tu’i Malila deep-sea hydrothermal vent fields along the Eastern Lau Spreading Center. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strains SG7T, SG1 and SLH were affiliated with the genus Methanocaldococcus within the family Methanocaldococcaceae , order Methanococcales . They shared 95.5–99.48 % 16S rRNA gene sequence similarity to other Methanocaldococcus species and were most closely related to Methanocaldococcus bathoardescens . Cells of strains SG7T, SG1 and SLH were cocci, with a diameter of 1.0–2.2 µm. The three strains grew between 45 and 93 °C (optimum, 80–85 °C), at pH 5.0–7.1 (optimum pH 6.2) and with 10–50 g l−1 NaCl (optimum 20–25 g l−1). Genome analysis revealed the presence of a 5.1 kbp plasmid in strain SG7T. Based on the results of average nucleotide identity and digital DNA–DNA hybridization analyses, we propose that strains SG1 and SG7T are representatives of a novel species, for which the name Methanocaldococcus lauensis sp. nov. is proposed; the type strain is SG7T (=DSM 109608T=JCM 39049T). 
PY 2023
SO International Journal Of Systematic And Evolutionary Microbiology
SN 1466-5026
PU Microbiology Society
VL 73
IS 1
UT 001008263300012
DI 10.1099/ijsem.0.005646
ID 92988
ER

EF