TY - JOUR T1 - Not out of the Mediterranean: Atlantic populations of the gorgonian Paramuricea clavata are a separate sister species under further lineage diversification A1 - Coelho,Márcio A. G. A1 - Pearson,Gareth A. A1 - Boavida,Joana R. H. A1 - Paulo,Diogo A1 - Aurelle,Didier A1 - Arnaud‐haond,Sophie A1 - Gómez‐gras,Daniel A1 - Bensoussan,Nathaniel A1 - López‐sendino,Paula A1 - Cerrano,Carlo A1 - Kipson,Silvija A1 - Bakran‐petricioli,Tatjana A1 - Ferretti,Eliana A1 - Linares,Cristina A1 - Garrabou,Joaquim A1 - Serrão,Ester A. A1 - Ledoux,Jean‐baptiste AD - Centre for Marine Sciences (CCMAR) University of Algarve Faro ,Portugal AD - MARE – Marine and Environmental Sciences Centre ISPA‐Instituto Universitário Lisboa,Portugal AD - Aix Marseille Univ., Université de Toulon, CNRS, IRD, MIO Marseille, France AD - Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National d'Histoire Naturelle, CNRS Sorbonne Université Paris ,France AD - MARBEC (Marine Biodiversity, Exploitation and Conservation) Univ. Montpellier, IFREMER, CNRS, IRD Sète Cedex ,France AD - Hawai‘i Institute of Marine Biology University of Hawai‘i at Mānoa Kaneohe Hawaii, USA AD - Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals Universitat de Barcelona (UB) Barcelona, Spain AD - Institut de Recerca de la Biodiversitat (IRBio) Universitat de Barcelona (UB) Barcelona ,Spain AD - Departament de Biologia Marina Institut de Ciències del Mar (CSIC) Barcelona, Spain AD - Dipartimento di Scienze della Vita e dell’Ambiente (DiSVA) Università Politecnica delle Marche Ancona ,Italy AD - Consorzio Nazionale Interuniversitario per le Scienze del Mare (CoNISMa) Rome, Italy AD - Stazione Zoologica Anton Dohrn, Department of Integrative Marine Ecology Ischia Marine Centre Naples, Italy AD - Fano Marine Center Fano ,Italy AD - Department of Biology, Faculty of Science University of Zagreb Zagreb ,Croatia AD - SEAFAN – Marine Research & Consultancy Zagreb, Croatia AD - Studio Associato GAIA s.n.c. Genoa, Italy AD - Institute of Marine Science The University of Auckland Auckland ,New Zealand AD - CIBIO/InBIO‐Centro de Investigação em Biodiversidade e Recursos Genéticos Vairão ,Portugal AD - CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental Universidade do Porto Porto ,Portugal UR - https://archimer.ifremer.fr/doc/00818/93037/ DO - 10.1002/ece3.9740 KW - Atlantic-Mediterranean transition KW - cryptic diversity KW - incomplete lineage sorting KW - Octocorallia KW - phylotranscriptomics KW - species delimitation N2 - The accurate delimitation of species boundaries in nonbilaterian marine taxa is notoriously difficult, with consequences for many studies in ecology and evolution. Anthozoans are a diverse group of key structural organisms worldwide, but the lack of reliable morphological characters and informative genetic markers hampers our ability to understand species diversification. We investigated population differentiation and species limits in Atlantic (Iberian Peninsula) and Mediterranean lineages of the octocoral genus Paramuricea previously identified as P. clavata. We used a diverse set of molecular markers (microsatellites, RNA-seq derived single-copy orthologues [SCO] and mt-mutS [mitochondrial barcode]) at 49 locations. Clear segregation of Atlantic and Mediterranean lineages was found with all markers. Species-tree estimations based on SCO strongly supported these two clades as distinct, recently diverged sister species with incomplete lineage sorting, P. cf. grayi and P. clavata, respectively. Furthermore, a second putative (or ongoing) speciation event was detected in the Atlantic between two P. cf. grayi color morphotypes (yellow and purple) using SCO and supported by microsatellites. While segregating P. cf. grayi lineages showed considerable geographic structure, dominating circalittoral communities in southern (yellow) and western (purple) Portugal, their occurrence in sympatry at some localities suggests a degree of reproductive isolation. Overall, our results show that previous molecular and morphological studies have underestimated species diversity in Paramuricea occurring in the Iberian Peninsula, which has important implications for conservation planning. Finally, our findings validate the usefulness of phylotranscriptomics for resolving evolutionary relationships in octocorals. Y1 - 2023/01 PB - Wiley JF - Ecology And Evolution SN - 2045-7758 VL - 13 IS - 1 ID - 93037 ER -