FN Archimer Export Format PT J TI Interlaboratory evaluation of multiple LC-MS/MS methods and a commercial ELISA method for determination of tetrodotoxin in oysters and mussels BT AF Turner, Andrew D Dean, Karl J Dhanji-Rapkova, Monika Dall’Ara, Sonia Pino, Florella McVey, Claire Haughey, Simon Logan, Natasha Elliott, Christopher Gago-Martinez, Ana Leao, Jose Manuel Giraldez, Jorge Gibbs, Ryan Thomas, Krista Perez-Calderon, Ruth Faulkner, Dermot McEneny, Hugh Savar, Veronique Reveillon, Damien Hess, Philipp Arevalo, Fabiola Lamas, J Pablo Cagide, Eva Alvarez, Mercedes Antelo, Alvaro Klijnstra, Mirjam D Oplatowska-Stachowiak, Michalina Kleintjens, Tim Sajic, Nermin Boundy, Michael J Maskrey, Benjamin H Harwood, D Tim Jartín, Jesús M González Alfonso, Amparo Botana, Luis AS 1:1;2:1;3:1;4:2;5:2;6:3;7:3;8:3;9:3;10:4;11:4;12:4;13:5;14:6;15:6;16:7;17:7;18:8;19:8;20:8;21:9;22:9;23:10;24:10;25:10;26:11;27:12;28:12;29:12;30:13;31:14;32:15;33:16;34:16;35:16; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:;17:;18:PDG-ODE-PHYTOX-METALG;19:PDG-ODE-PHYTOX-METALG;20:PDG-ODE-PHYTOX;21:;22:;23:;24:;25:;26:;27:;28:;29:;30:;31:;32:;33:;34:;35:; C1 Centre for Environment, Fisheries and Aquaculture Science , Barrack Road, The Nothe, Weymouth, Dorset, DT4 8UB United Kingdom Fondazione Centro Ricerche Marine , Viale A. Vespucci, 2, 47042 kleintjens FC, Italy Queen’s University Belfast, Institute for Global Food Security , 19 Chlorine Gardens, Belfast, BT9 5DL United Kingdom University of Vigo , Department of Chemistry, Bloque E, Planta 2, Despacho 17, As Lagoas, Marcosende, 36310 Vigo, Spain Canadian Food Inspection Agency , 1992 Agency Drive, Dartmouth, NS, B3B 1Y9 Canada National Research Council of Canada , Biotoxin Metrology, 1411 Oxford Street, Halifax, NS, B3H 3Z1 Canada Agri-Food and Biosciences Institute, Veterinary Sciences Division , Stoney Road, Belfast, United Kingdom, BT4 3SD Ifremer, PHYTOX Unit , F-44000 Nante, s, France Instituto Tecnoloxico para o Control do Medio Mariño de Galicia , Peirao de Vilaxoan, s/n, 36611 Vilagarcia de Arousa, Pontevedra, Spain Laboratorio Cifga, Avenida Benigno Rivera , No. 56, 27003 Lugo, Spain Wageningen Food Safety Research, Wageningen University and Research , Akkermaalsbos 2, 6708WB Wageningen, The Netherlands R-Biopharm Nederland B.V , Beijerinckweg 18, 6827 BN Arnhem, The Netherlands Cawthron Institute , 98 Halifax Street East, Nelson 7010, Private Bag 2, Nelson, 7042 New Zealand CTL Ltd , Pakefield Road, Lowestoft, Suffolk, NR33 0HT United Kingdom CNC , 3 Elms Street, Stoke, 7011 New Zealand Universidade De Santiago De Compostela Deparamento de Farmacologa, Facultad de Veterinaria, , 27002 Lugo, Spain C2 CEFAS, UK CRM, ITALY UNIV BELFAST, UK UNIV VIGO, SPAIN CANADIAN FOOD INSPECTION AGCY, CANADA NATL RES COUNCIL CANADA, CANADA AFBI, UK IFREMER, FRANCE INTECMAR, SPAIN LABORATORIO CIFGA, SPAIN UNIV WAGENINGEN, NETHERLANDS R-BIOPHARM NEDERLAND B.V, NETHERLANDS CAWTHRON INST, NEW ZEALAND CTL LTD, UK CNC, NEW ZEALAND UNIV SANTIAGO DE COMPOSTELA, SPAIN SI NANTES SE PDG-ODE-PHYTOX-METALG PDG-ODE-PHYTOX IN WOS Ifremer UPR copubli-europe copubli-int-hors-europe IF 1.6 TC 2 UR https://archimer.ifremer.fr/doc/00819/93065/99518.pdf https://archimer.ifremer.fr/doc/00819/93065/99519.zip LA English DT Article AB Background Given the recent detection of TTX in bivalve molluscs but the absence of a full collaborative validation study for TTX determination in a large number of shellfish samples, interlaboratory assessment of method performance was required to better understand current capabilities for accurate and reproducible TTX quantitation using chemical and immunoassay methods. Objective The aim was to conduct a collaborative study with multiple laboratories, using results to assess method performance and acceptability of different TTX testing methods Methods Homogenous and stable mussel and oyster materials were assessed by participants using a range of published and in-house detection methods to determine mean TTX concentrations. Data was used to calculate recoveries, repeatability and reproducibility, together with participant acceptability z-scores. Results Method performance characteristics were good, showing excellent sensitivity, recovery and repeatability. Acceptable reproducibility was evidenced by HorRat values for all LC-MS/MS and ELISA methods being less than the 2.0 limit of acceptability. Method differences between the LC-MS/MS participants did not result in statistically-different results. Method performance characteristics compared well with previously-published single-laboratory validated methods and no statistical difference was found in results returned by ELISA in comparison with LC-MS/MS. Conclusions The results from this study demonstrate that current LC-MS/MS methods and the ELISA are on the whole capable of sensitive, accurate and reproducible TTX quantitation in shellfish. Further work is recommended to expand the number of laboratories testing ELISA and to standardise an LC-MS/MS protocol to further improve interlaboratory precision. Highlights Multiple mass spectrometric methods and a commercial ELISA have been successfully assessed through collaborative study, demonstrating excellent performance. PY 2023 PD MAR SO Journal Of Aoac International SN 1060-3271 PU Oxford University Press (OUP) VL 106 IS 2 UT 000920320700001 BP 356 EP 369 DI 10.1093/jaoacint/qsad006 ID 93065 ER EF