FN Archimer Export Format
PT J
TI High‐resolution phylogenetic analysis reveals long‐term microbial dynamics and microdiversity in phytoplankton microbiome
BT 
AF Choi, Chang Jae
   Jauzein, Cecile
   Erdner, Deana L.
AS 1:1,2;2:1,3;3:1;
FF 1:;2:PDG-ODE-DYNECO-PELAGOS;3:;
C1 The University of Texas at Austin Marine Science Institute Port Aransas TX, USA
   Fort Lauderdale Research and Education Center, University of Florida, Davie, FL, USA
   Ifremer, Dynamiques des Ecosystèmes Côtiers (DYNECO), Laboratoire d’Ecologie Pélagique (PELAGOS) Plouzané ,France
C2 UNIV TEXAS AUSTIN, USA
   UNIV FLORIDA, USA
   IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer UPR
   copubli-int-hors-europe
IF 2.2
TC 1
UR https://archimer.ifremer.fr/doc/00820/93216/99857.pdf
LA English
DT Article
DE ;16S rRNA gene V4 amplicon sequencing;Alexandrium;phytoplankton-bacteria interactions;Roseobacter
AB Phytoplankton-bacteria interactions represent the evolution of complex cross-kingdom networks requiring niche specialization of diverse microbes. Unraveling this co-evolutionary process has proven challenging because microbial partnerships are complex, and their assembly can be dynamic as well as scale- and taxon-dependent. Here we monitored long-term experimental evolution of phytoplankton-bacteria interactions by reintroducing the intact microbiome into an axenized dinoflagellate Alexandrium tamarense to better understand microbiome assembly dynamics and how microbiome composition could shift and stabilize over 15 months. We examined host functioning by growth rate, photosynthetic capability, cell size, and other physiological signatures and compared it to associated microbial communities determined by 16S rRNA gene sequences. Our results showed that microbiome reconstitution did not restore the intact microbiome, instead a distinct microbial community shift to Roseobacter clade was observed in the re-established cultures. In-depth comparisons of microbial interactions revealed no apparent coupling between host physiology and specific bacterial taxa, indicating that highly represented, abundant taxa might not be essential for host functioning. The emergence of highly divergent Roseobacter clade sequences suggests fine-scale microbial dynamics driven by microdiversity could be potentially linked to host functioning. Collectively, our results indicate that functionally comparable microbiomes can be assembled from markedly different, highly diverse bacterial taxa in changing environments. 
PY 2023
PD MAY
SO Journal Of Eukaryotic Microbiology
SN 1066-5234
PU Wiley
VL 70
IS 3
UT 000942186700001
DI 10.1111/jeu.12966
ID 93216
ER

EF