FN Archimer Export Format
PT J
TI The Anti-Listeria Activity of Pseudomonas fluorescens Isolated from the Horticultural Environment in New Zealand
BT 
AF Mohan, Vanessa
   Wibisono, Reginald
   Chalke, Saili
   Fletcher, Graham
   LEROI, Francoise
AS 1:1;2:1;3:1;4:1;5:2;
FF 1:;2:;3:;4:;5:PDG-RBE-MASAE-LEMMMB;
C1 The New Zealand Institute for Plant and Food Research, Private Bag, Auckland 92169, New Zealand
   IFREMER, MASAE Microbiologie Aliment Santé Environnement, F-44000 Nantes, France
C2 NEW ZEALAND INST PLANT FOOD RES, NEW ZEALAND
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-MASAE-LEMMMB
IN WOS Ifremer UPR
   DOAJ
   copubli-int-hors-europe
IF 3.7
TC 1
UR https://archimer.ifremer.fr/doc/00821/93299/99990.pdf
LA English
DT Article
DE ;Pseudomonas fluorecens;Listeria spp;biocontrol agents;tss-T6SS secretion system;anti-listeria activity;structural proteome analysis
AB Beneficial bacteria with antibacterial properties are attractive alternatives to chemical-based antibacterial or bactericidal agents. Our study sourced such bacteria from horticultural produce and environments to explore the mechanisms of their antimicrobial properties. Five strains of Pseudomonas fluorescens were studied that possessed antibacterial activity against the pathogen Listeria monocytogenes. The vegetative culture of these strains (Pseudomonas fluorescens-PFR46I06, Pseudomonas fluorescens-PFR46H06, Pseudomonas fluorescens-PFR46H07, Pseudomonas fluorescens-PFR46H08 and Pseudomonas fluorescens-PFR46H09) were tested against Listeria monocytogenes (n = 31), Listeria seeligeri (n = 1) and Listeria innocua (n = 1) isolated from seafood and horticultural sources and from clinical cases (n = 2) using solid media coculture and liquid media coculture. All Listeria strains were inhibited by all strains of P. fluorescens; however, P. fluorescens-PFR46H07, P. fluorescens-PFR46H08 and P. fluorescens-PFR46H09 on solid media showed good inhibition, with average zones of inhibition of 14.8 mm, 15.1 mm and 18.2 mm, respectively, and the other two strains and P. fluorescens-PFR46H09 had a significantly greater zone of inhibition than the others (p < 0.05). There was no inhibition observed in liquid media coculture or in P. fluorescens culture supernatants against Listeria spp. by any of the P. fluorescens strains. Therefore, we hypothesized that the structural apparatus that causes cell-to-cell contact may play a role in the ejection of ant-listeria molecules on solid media to inhibit Listeria isolates, and we investigated the structural protein differences using whole-cell lysate proteomics. We paid special attention to the type VI secretion system (TSS-T6SS) for the transfer of effector proteins or bacteriocins. We found significant differences in the peptide profiles and protein summaries between these isolates’ lysates, and PFR46H06 and PFR46H07 possessed the fewest secretion system structural proteins (12 and 11, respectively), while PFR46H08 and PFR46H09 had 18 each. P. fluorescens-PFR46H09, which showed the highest antimicrobial effect, had nine tss-T6SS structural proteins compared to only four in the other three strains. 
PY 2023
PD FEB
SO Pathogens
SN 2076-0817
PU MDPI
VL 12
IS 2
UT 000941718100001
DI 10.3390/pathogens12020349
ID 93299
ER

EF