FN Archimer Export Format
PT J
TI When phytoplankton do not bloom: the case of the dinoflagellate Lepidodinium chlorophorum in southern Brittany (France) assessed by environmental DNA
BT 
AF Roux, Pauline
   Schapira, Mathilde
   MERTENS, Kenneth
   ANDRE, Coralie
   TERRE TERRILLON, Aouregan
   Schmitt, Anne
   Manach, Soazig
   Collin, Karine
   Serghine, Joelle
   Noel, Cyril
   Siano, Raffaele
AS 1:1;2:1;3:2;4:2;5:2;6:1;7:3;8:1;9:4;10:5;11:4;
FF 1:PDG-ODE-LITTORAL-LERMPL;2:PDG-ODE-LITTORAL-LERMPL;3:PDG-ODE-LITTORAL-LERBO;4:;5:PDG-ODE-LITTORAL-LERBO;6:PDG-ODE-LITTORAL-LERMPL;7:PDG-ODE-LITTORAL-LERMPL;8:PDG-ODE-LITTORAL-LERMPL;9:PDG-ODE-DYNECO-PELAGOS;10:PDG-IRSI-SEBIMER;11:PDG-ODE-DYNECO-PELAGOS;
C1 Ifremer, LITTORAL, F-44300 Nantes, France
   Ifremer, LITTORAL, F-29900 Concarneau, France
   Ifremer, LITTORAL, F-56100 Lorient, France
   Ifremer, DYNECO, F-29280 Plouzané, France
   Ifremer, IRSI, Service de Bioinformatique (SeBiMER), Plouzané, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   CONCARNEAU
   LORIENT
   BREST
SE PDG-ODE-LITTORAL-LERMPL
   PDG-ODE-LITTORAL-LERBO
   PDG-ODE-DYNECO-PELAGOS
   PDG-IRSI-SEBIMER
IN WOS Ifremer UPR
IF 4.1
TC 2
UR https://archimer.ifremer.fr/doc/00822/93374/100079.pdf
LA English
DT Article
DE ;HABs;Cysts;Sediment resuspension;eDNA;Metabarcoding;Ammonium
AB Green seawater discolorations caused by the marine dinoflagellate Lepidodinium chlorophorum are frequently observed during summer along southern Brittany coasts (NE Atlantic, France). Here, the ecology of L. chlorophorum is studied during a non-bloom period using high-throughput sequencing metabarcoding of environmental DNA (eDNA) samples for the detection of this species at low concentrations. Sediment samples (for metabarcoding and cyst analyses) were collected in January–February 2019 and water samples from two stations were collected at three water depths in September–March 2019–2020 and 2020–2021 (for metabarcoding and environmental parameters). The protistan community was dominated by dinoflagellates and was homogenous in the water column. Amplicon sequence variants (ASVs) associated with the genus Lepidodinium were detected in autumn–winter at low relative abundances (minimum: 0.01%). Increases in Lepidodinium abundance were positively correlated with pulses of ammonium re-suspended from bottom sediments. Although Lepidodinium eDNA (<1%) was detected in the sediments, no cyst morphotypes could be associated with Lepidodinium, and germination experiments revealed no Lepidodinium-like cells, leaving in doubt the existence of resting cysts of this species in the seed bank. It is hypothesised that temporary Lepidodinium cells remained present in the water column at low concentrations during the autumn–winter period, awaiting ammonium input from sediments to initiate growth, and that blooms develop when water column stratification and river input provide favourable environmental conditions for biomass increases. 
PY 2023
PD MAR
SO Progress In Oceanography
SN 0079-6611
PU Elsevier BV
VL 212
UT 000952484600001
DI 10.1016/j.pocean.2023.102999
ID 93374
ER

EF