FN Archimer Export Format
PT J
TI Mortality and histopathology in sheepshead minnow (Cyprinodon variegatus) larvae exposed to pectenotoxin-2 and Dinophysis acuminata
BT 
AF Gaillard, Sylvain
   Réveillon, Damien
   Mason, P.L.
   Ayache, Nour
   Sanderson, M.
   Smith, J.L.
   Giddings, S.
   McCarron, P.
   Séchet, Veronique
   Hégaret, Helene
   Hess, Philipp
   Vogelbein, W.K.
AS 1:1,2;2:2;3:1;4:1;5:1;6:1;7:3;8:3;9:2;10:4;11:2;12:1;
FF 1:PDG-ODE-DYNECO-PHYC;2:PDG-ODE-PHYTOX-METALG;3:;4:;5:;6:;7:;8:;9:PDG-ODE-PHYTOX-PHYSALG;10:;11:PDG-ODE-PHYTOX;12:;
C1 Virginia Institute of Marine Science, William & Mary, P.O. Box 1346, Gloucester Point, VA 23062, USA
   IFREMER, PHYTOX unit, F-44000 Nantes, France
   Biotoxin Metrology, National Research Council Canada, 1411 Oxford Street, Nova Scotia, Halifax B3H 3Z1, Canada
   Laboratoire des Sciences de l'Environnement Marin (UMR6539 CNRS/UBO/IFREMER/IRD), Institut Universitaire Européen de la Mer, Technopôle Brest-Iroise, Plouzané 29280, France
C2 VIRGINIA INST MARINE SCI, USA
   IFREMER, FRANCE
   NATL RES COUNCIL CANADA, CANADA
   CNRS, FRANCE
SI NANTES
SE PDG-ODE-DYNECO-PHYC
   PDG-ODE-PHYTOX-METALG
   PDG-ODE-PHYTOX-PHYSALG
   PDG-ODE-PHYTOX
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   copubli-france
   copubli-int-hors-europe
IF 4.5
TC 3
UR https://archimer.ifremer.fr/doc/00822/93415/100145.pdf
LA English
DT Article
DE ;Dinoflagellate;Fish;Gills;Harmful algal bloom;Histopathology;Pectenotoxin;Dinophysis
AB Toxic species of the dinoflagellate genus Dinophysis can produce diarrheic toxins including okadaic acid (OA) and dinophysistoxins (DTXs), and the non-diarrheic pectenotoxins (PTXs). Okadaic acid and DTXs cause diarrheic shellfish poisoning (DSP) in human consumers, and also cause cytotoxic, immunotoxic and genotoxic effects in a variety of mollusks and fishes at different life stages in vitro. The possible effects of co-produced PTXs or live cells of Dinophysis to aquatic organisms, however, are less understood. Effects on an early life stage of sheepshead minnow (Cyprinodon variegatus), a common finfish in eastern USA estuaries, were evaluated using a 96-h toxicity bioassay. Three-week old larvae were exposed to PTX2 concentrations from 50 to 4000 nM, live Dinophysis acuminata culture (strain DAVA01), live cells resuspended in clean medium or culture filtrate. This D. acuminata strain produced mainly intracellular PTX2 (≈ 21 pg cell−1), with much lower levels of OA and dinophysistoxin-1. No mortality or gill damages were observed in larvae exposed to D. acuminata (from 5 to 5500 cells mL−1), resuspended cells and culture filtrate. However, exposure to purified PTX2 at intermediate to high concentrations (from 250 to 4000 nM) resulted in 8 to 100% mortality after 96 h (24-h LC50 of 1231 nM). Histopathology and transmission electron microscopy of fish exposed to intermediate to high PTX2 concentrations revealed important gill damage, including intercellular edema, necrosis and sloughing of gill respiratory epithelia, and damage to the osmoregulatory epithelium, including hypertrophy, proliferation, redistribution and necrosis of chloride cells. Tissue damage in gills is likely caused by the interaction of PTX2 with the actin cytoskeleton of the affected gill epithelia. Overall, the severe gill pathology observed following the PTX2 exposure suggested death was due to loss of respiratory and osmoregulatory functions in C. variegatus larvae. 
PY 2023
PD APR
SO Aquatic Toxicology
SN 0166-445X
PU Elsevier BV
VL 257
UT 000953527400001
DI 10.1016/j.aquatox.2023.106456
ID 93415
ER

EF