FN Archimer Export Format
PT J
TI Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas.
BT 
AF Sol Dourdin, Thomas
   Rivière, Guillaume
   Cormier, Alexandre
   DI POI BROUSSARD, Carole
   Guyomard, Killian
   Rabiller, Manuella
   Akcha, Farida
   BAH, Thierno Sadialiou
   Le Monier, Pauline
   Sussarellu, Rossana
AS 1:1;2:2;3:3;4:4;5:5;6:4;7:1;8:1;9:1;10:6;
FF 1:PDG-RBE-CCEM;2:;3:PDG-IRSI-SEBIMER;4:PDG-RBE-PHYTNESS;5:PDG-RBE-EMMA-PMMB;6:PDG-RBE-EMMA-PMMB;7:PDG-RBE-CCEM;8:;9:PDG-RBE-CCEM;10:PDG-ODE-PHYTOX-GENALG;
C1 Ifremer, Unité Contamination Chimique des Ecosystèmes Marins, Nantes, France
   Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), UMR8067, Muséum National D’Histoire Naturelle (MNHN), Centre National de La Recherche Scientifique (CNRS), Institut de Recherche et Développement (IRD), Sorbonne Université (SU), Université de Caen Normandie (UCN), Université des Antilles (UA), 75231, Paris, CEDEX, France
   Ifremer, Service de Bioinformatique de L’Ifremer, Brest, France
   Laboratoire des Sciences de L'environnement Marin (LEMAR), France
   Ifremer, Plateforme Mollusques Marins Bouin, Bouin, France
   Ifremer, Physiologie et Toxines des Microalgues Toxiques, Nantes, France
C2 IFREMER, FRANCE
   MNHN, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI NANTES
   BREST
   ARGENTON
   BOUIN
SE PDG-RBE-CCEM
   PDG-IRSI-SEBIMER
   PDG-RBE-PHYTNESS
   PDG-RBE-EMMA-PMMB
   PDG-RBE-BE-LEX
   PDG-ODE-PHYTOX-GENALG
UM LEMAR
IN WOS Ifremer UPR
   WOS Ifremer UMR
   copubli-france
IF 8.9
TC 4
UR https://archimer.ifremer.fr/doc/00829/94090/101184.pdf
LA English
DT Article
DE ;Mixture;Epigenetics;Gene expression;Molluscs;Pesticides;DNA methylation
AB Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecological characteristics (benthic, sessile, filter feeding). So far studies have investigated the impact of xenobiotics at embryotoxic, genotoxic and physiological endpoints, sometimes at the multigeneration scale, highlighting the role of epigenetic mechanisms in transmitting alterations induced by exposure to single xenobiotics. However, to date, little is known about the impact of environmentally-mimicking contaminants cocktails. Thus, we examined the impact of an early exposure to environmentally relevant mixture on the Pacific oyster life history. We studied transcriptomic, epigenetic and physiological alterations induced in oysters exposed to 18 pesticides and metals at environmental concentration (nominal sum concentration: 2.85 μg.L−1, measured sum concentration: 3.74 ± 0.013 μg.L−1) during embryo-larval stage (0–48 h post fertilization, hpf). No significant differences in embryo-larval abnormalities at 24 hpf were observed during larval and spat rearing; the swimming behaviour of exposed individuals was disturbed, while they were longer and heavier at specific time points, and exhibited a lower epinephrine-induced metamorphosis rate as well as a higher survival rate in the field. In addition, RNA-seq analyses of gastrula embryos revealed the differential expression of development-related genes (e.g. Hox orthologues and cell cycle regulators) between control and exposed oysters. Whole-genome DNA methylation analyses demonstrated a significant modification of DNA methylation in exposed larvae marked by a demethylation trend. Those findings suggest that early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life history traits in the Pacific oyster. 
PY 2023
PD JUL
SO Environmental Pollution
SN 0269-7491
PU Elsevier BV
VL 326
UT 000971242100001
DI 10.1016/j.envpol.2023.121472
ID 94090
ER

EF