FN Archimer Export Format
PT J
TI Optimization of R-Phycoerythrin Extraction by Ultrasound-Assisted Enzymatic Hydrolysis: A Comprehensive Study on the Wet Seaweed Grateloupia turuturu
BT 
AF LE GUILLARD, Cécile
   BERGÉ, Jean-Pascal
   DONNAY-MORENO, Claire
   CORNET, Josiane
   RAGON, Jean-Yves
   FLEURENCE, Joel
   DUMAY, Justine
AS 1:1,2;2:3;3:1;4:1;5:1;6:2;7:2;
FF 1:;2:;3:PDG-RBE-MASAE-LEMMMB;4:PDG-RBE-BRM-LEMMMB;5:PDG-RBE-BRM-EM3B;6:;7:;
C1 IFREMER Centre Ifremer Atlantique, EM3B, BP 21105, CEDEX 03, 44311 Nantes, France
   Institut des Substances et Organismes de la Mer, ISOMER, Nantes Université, UR 2160, 44000 Nantes, France
   UPCYCLINK, 22 Rue de Mangorvenec, 56890 Saint-Avé, France
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   UPCYCLINK, FRANCE
SI NANTES
SE PDG-RBE-MASAE-LEMMMB
   PDG-RBE-BRM-LEMMMB
   PDG-RBE-BRM-EM3B
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
IF 5.4
TC 0
UR https://archimer.ifremer.fr/doc/00830/94159/101412.pdf
LA English
DT Article
DE ;seaweed;Grateloupia turuturu;R-phycoerythrin;ultrasound-assisted enzymatic hydrolysis (UAEH);extraction process;response surface methodology
AB Enzyme-assisted extraction (EAE) and ultrasound-assisted extraction (UAE) are both recognized as sustainable processes, but little has been done on the combined process known as ultrasound-assisted enzymatic hydrolysis (UAEH), and even less on seaweed. The present study aimed to optimize the UAEH of the red seaweed Grateloupia turuturu for the extraction of R-phycoerythrin (R-PE) directly from the wet biomass by applying a response surface methodology based on a central composite design. Three parameters were studied: the power of ultrasound, the temperature and the flow rate in the experimental system. Data analysis demonstrated that only the temperature had a significant and negative effect on the R-PE extraction yield. Under the optimized conditions, the R-PE kinetic yield reached a plateau between 90 and 210 min, with a yield of 4.28 ± 0.09 mg·g−1 dry weight (dw) at 180 min, corresponding to a yield 2.3 times higher than with the conventional phosphate buffer extraction on freeze-dried G. turuturu. Furthermore, the increased release of R-PE, carbohydrates, carbon and nitrogen can be associated with the degradation of G. turuturu constitutive polysaccharides, as their average molecular weights had been divided by 2.2 in 210 min. Our results thus demonstrated that an optimized UAEH is an efficient method to extract R-PE from wet G. turuturu without the need for expensive pre-treatment steps found in the conventional extraction. UAEH represents a promising and sustainable approach that should be investigated on biomasses where the recovery of added-value compounds needs to be improved. 
PY 2023
PD APR
SO Marine Drugs
SN 1660-3397
PU MDPI
VL 21
IS 4
UT 000977109300001
DI 10.3390/md21040213
ID 94159
ER

EF