FN Archimer Export Format PT J TI Mission Tara Microplastics: a holistic set of protocols and data resources for the field investigation of plastic pollution along the land-sea continuum in Europe BT AF Ghiglione, Jean-François Barbe, Valérie Bruzaud, Stéphane Burgaud, Gaëtan Cachot, Jerome Eyheraguibel, Boris Lartaud, Franck Ludwig, Wolfgang Meistertzheim, Anne-Leila Paul PONT, Ika Pesant, Stéphane ter Halle, Alexandra Thiebeauld, Odon AS 1:1,2;2:3;3:4;4:5;5:6;6:7;7:8;8:9;9:10;10:11;11:2,12;12:13;13:14;14:; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:; C1 CNRS, Sorbonne Université, Laboratoire d’Océanographie Microbienne (LOMIC)/UMR 7621, Observatoire Océanologique de Banyuls, Laboratoire d’Océanographie Microbienne, 1 Avenue Fabre, F-66650, Banyuls sur mer, France Research Federation for the Study of Global Ocean Systems Ecology and Evolution, R2022/Tara Oceans-GOSEE, Paris, France Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, Evry, France UMR CNRS 6027, IRDL, Université Bretagne Sud, 56100, Lorient, France Univ Brest, INRAE, Laboratoire Universitaire de Biodiversité Et Écologie Microbienne, 29280, Plouzané, France Université Bordeaux, EPOC CNRS, EPHE, Université de Bordeaux, UMR 5805, 33600, Pessac, France CNRS, Université Clermont Auvergne, Institut de Chimie de Clermont-Ferrand (ICCF), UMR6296, Clermont-Ferrand, France CNRS, Sorbonne Université, Laboratoire d’Ecogéochimie des Environnements Benthiques (LECOB)/UMR 8222, Observatoire Océanologique de Banyuls, Banyuls Sur Mer, France CEFREM, UMR 5110, University of Perpignan - CNRS, 66860, Perpignan Cedex, France SAS Plastic@Sea, Observatoire Océanologique de Banyuls, Banyuls Sur Mer, France Ifremer, CNRS, IRD, LEMAR, Univ Brest, F‐29280, Plouzané, France European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SD, UK CNRS, Laboratoire des InteractionsMoléculaires EtRéactivité Chimique Et Photochimique (IMRCP), UMR 5623, Université de Toulouse, Toulouse, France ImmunRise Biocontrol France, Cestas, France C2 CNRS, FRANCE TARA OCEANS GOSEE, FRANCE UNIV PARIS SACLAY, FRANCE UBS, FRANCE UBO, FRANCE UNIV BORDEAUX, FRANCE CNRS, FRANCE CNRS, FRANCE UNIV PERPIGNAN, FRANCE SAS PLASTIC@SEA, FRANCE IFREMER, FRANCE EMBL, UK CNRS, FRANCE IMMUNRISE BIOCONTROL FRANCE, FRANCE SI BREST TAHITI TOULON SE PDG-REM-EEP-LMEE PDG-IRSI-ISI PDG-IRSI-RIC IRD PDG-RBE-PHYTNESS PDG-RBE-RMPF PDG-ODE-LITTORAL-LERPAC UM BEEP-LM2E LEMAR EIO TC 0 UR https://archimer.ifremer.fr/doc/00835/94738/102212.pdf LA English DT Article DE ;Microplastics;Land-sea continuum;Toxicology;Plastisphere;Scientific expedition AB Abstract The Tara Microplastics mission was conducted for 7 months to investigate plastic pollution along nine major rivers in Europe—Thames, Elbe, Rhine, Seine, Loire, Garonne, Ebro, Rhone, and Tiber. An extensive suite of sampling protocols was applied at four to five sites on each river along a salinity gradient from the sea and the outer estuary to downstream and upstream of the first heavily populated city. Biophysicochemical parameters including salinity, temperature, irradiance, particulate matter, large and small microplastics (MPs) concentration and composition, prokaryote and microeukaryote richness, and diversity on MPs and in the surrounding waters were routinely measured onboard the French research vessel Tara or from a semi-rigid boat in shallow waters. In addition, macroplastic and microplastic concentrations and composition were determined on river banks and beaches. Finally, cages containing either pristine pieces of plastics in the form of films or granules, and others containing mussels were immersed at each sampling site, 1 month prior to sampling in order to study the metabolic activity of the plastisphere by meta-OMICS and to run toxicity tests and pollutants analyses. Here, we fully described the holistic set of protocols designed for the Mission Tara Microplastics and promoted standard procedures to achieve its ambitious goals: (1) compare traits of plastic pollution among European rivers, (2) provide a baseline of the state of plastic pollution in the Anthropocene, (3) predict their evolution in the frame of the current European initiatives, (4) shed light on the toxicological effects of plastic on aquatic life, (5) model the transport of microplastics from land towards the sea, and (6) investigate the potential impact of pathogen or invasive species rafting on drifting plastics from the land to the sea through riverine systems. PY 2023 PD MAY SO Environmental Science and Pollution Research SN 1614-7499 PU Springer Science and Business Media LLC DI 10.1007/s11356-023-26883-9 ID 94738 ER EF