FN Archimer Export Format
PT J
TI Genotype x nutrition interactions in European sea bass (Dicentrarchus labrax): Effects on gut health and intestinal microbiota
BT 
AF Torrecillas, S.
   Rimoldi, S.
   Montero, D.
   Serradell, A.
   Acosta, F.
   Fontanillas, R.
   Allal, Francois
   Haffray, P.
   Bajek, A.
   Terova, G.
AS 1:1;2:1,2;3:1;4:1;5:1;6:3;7:4;8:5;9:6;10:2;
FF 1:;2:;3:;4:;5:;6:;7:PDG-RBE-MARBEC-LAAAS;8:;9:;10:;
C1 Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain
   Department of Biotechnology and Life Sciences, University of Insubria, Via J.H. Dunant, 3, 21100 Varese, Italy
   Skretting Aquaculture Research Centre, Stavanger, Norway
   MARBEC, University of Montpellier, CNRS, Ifremer, IRD, 34250 Palavas-les-Flots, France
   SYSAAF (French Poultry and Aquaculture Breeders Technical Centre), 35042 Rennes, France
   Ecloserie Marine de Graveline Ichtus, Route des Enrochements, 59820, Gravelines, France
C2 UNIV LAS PALMAS GRAN CANARIA, SPAIN
   UNIV INSUBRIA, ITALY
   SKRETTING ARC, NORWAY
   IFREMER, FRANCE
   SYSAAF, FRANCE
   ECLOSERIE MARINE GRAVELINES ICHTUS, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
UM MARBEC
IN WOS Ifremer UMR
   copubli-france
   copubli-europe
IF 4.5
TC 4
UR https://archimer.ifremer.fr/doc/00835/94741/102218.pdf
LA English
DT Article
DE ;Aquaculture;Future diets;GALT;Gut morphology;Microbiota
AB One of the main objectives of the present study was an effective replacement of dietary fishmeal/fish oil (FM/FO) by new raw materials without negatively affecting European sea bass (Dicentrarchus labrax) performance and health status within a selection breeding context. The genomic selection of this species is still in its infancy if compared to salmonids and in particular, the role of D.labrax genome in shaping the gut microbiome, has been scarcely investigated. Wildtype (WT) and selected (GS) sea bass were fed with two diets: a control (20% FM/ 5–9% FO), and a “future” (F) diet, in which FM was decreased to 10% being replaced by poultry meal, whereas FO was completely replaced by a blend of rapeseed, poultry, and algae oils. The morphological evaluation of the intestine revealed a well-organized folding pattern and a conserved gut epithelial barrier for all fish groups. Despite a basal level of inflammation in the proximal intestine of WT fish, no differences were observed neither in the morphometric characteristics of goblet cells nor in the expression of GALT-related genes in response to fish genotype or diet. At distal intestine, WT fish showed a higher inflammatory status and larger goblet cells than GS fish and within the same genotype, fish fed the F diet had in general larger goblet cells. In distal intestine, a significant effect was found on the expression of 3 out of 7 target GALT genes. In particular, the expression of cytokines il-1β, tnf-α, and il-10 was different, showing an interaction effect diet x genotype. Diet had a lower influence upon gut bacterial composition than genotype. Indeed, regardless of the diet, WT fish showed higher species richness than GS genotype and this could be a direct consequence of selective breeding for multiple traits selection including growth, external morphology for lower abdominal fat deposition and adaptation to multiple and successive feed sources and composition across generations of selection. Furthermore, the gut microbiota of GS fish shared a reduced individual variability, indicating an enhanced capacity to cope with changes in diet composition. The less changes of GS sea bass at the level of gut bacterial composition in cumulating data collected with the feeds, demonstrate a capacity to reshape their microbiota thus better adapting to the diet, but with no negative impact on their growth performances, and even a better growth. A significant genotype effect was found for specific bacterial taxa, such as Paracoccus genus and other genera belonging to Moraxellaceae family, which were enriched in WT fish, regardless of the diet. Interestingly, the relative abundance of Paracoccus genus was positively correlated with higher proinflammatory cytokine il-1β expression found in distal intestine of wildtype sea bass. 
PY 2023
PD SEP
SO Aquaculture
SN 0044-8486
PU Elsevier BV
VL 574
UT 001005032700001
DI 10.1016/j.aquaculture.2023.739639
ID 94741
ER

EF