FN Archimer Export Format PT J TI Cooperation and cheating orchestrate Vibrio assemblages and polymicrobial synergy in oysters infected with OsHV-1 virus BT AF Oyanedel, Daniel Lagorce, Arnaud Bruto, Maxime Haffner, Philippe Morot, Amandine DORANT, Yann de La Forest Divonne, Sebastien Delavat, François Inguimbert, Nicolas Montagnani, Caroline Morga, Benjamin Toulza, Eve Chaparro, Cristian Escoubas, Jean-Michel Labreuche, Yannick Gueguen, Yannick VIDAL DUPIOL, Jeremie de Lorgeril, Julien Petton, Bruno Degremont, Lionel Tourbiez, Delphine Pimparé, Léa-Lou Leroy, Marc Romatif, Oceane Pouzadoux, Juliette Mitta, Guillaume Roux, Frédérique Le Charrière, Guillaume M. Travers, Agnes Destoumieux-Garzón, Delphine AS 1:12;2:1;3:2,3;4:1;5:4,5;6:1;7:12;8:6;9:7;10:1;11:8;12:12;13:12;14:12;15:2;16:1,9;17:1;18:1,10;19:2,4;20:8;21:8;22:12;23:12;24:1;25:1;26:11,12;27:2,3;28:12;29:1;30:12; FF 1:;2:;3:PDG-RBE-PFOM;4:PDG-RBE-IHPE;5:;6:PDG-RBE-IHPE;7:;8:;9:;10:PDG-RBE-IHPE;11:PDG-RBE-ASIM;12:;13:;14:;15:PDG-RBE-PHYTNESS;16:PDG-ODE-LITTORAL-LERLR;17:PDG-RBE-IHPE;18:PDG-RBE-LEADNC;19:PDG-RBE-PHYTNESS;20:PDG-RBE-ASIM;21:PDG-RBE-ASIM;22:;23:;24:PDG-RBE-IHPE;25:PDG-RBE-IHPE;26:;27:;28:;29:PDG-RBE-IHPE;30:; C1 IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan. Montpellier, France Ifremer, Unité Physiologie Fonctionnelle des Organismes Marins, ZI de la Pointe du Diable, Plouzané, France Sorbonne Université, UPMC Paris 06, CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, Roscoff cedex, France Université de Bretagne Occidentale, CNRS, IRD, Ifremer, LEMAR, F-29280 Plouzané, France Université de Bretagne-Sud, EA 3884, LBCM, IUEM, F-56100 Lorient, France Nantes Université, CNRS, US2B, UMR6286, F-44000 Nantes, France CRIOBE, USR EPHE-UPVD-CNRS 3278, Université de Perpignan Via Domitia, 58 Avenue Paul Alduy, 66860 Perpignan, France Ifremer, ASIM, Adaptation Santé des invertébrés, La Tremblade, France MARBEC, Univ Montpellier, CNRS, Ifremer, IRD, Sète, France Ifremer, IRD, Univ Nouvelle-Calédonie, Univ La Réunion, ENTROPIE, F-98800, Nouméa, Nouvelle-Calédonie, France Ifremer, Université de Polynésie Française, IRD, ILM, EIO, Polynésie Française IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan. Montpellier, France C2 IFREMER, FRANCE IFREMER, FRANCE UNIV SORBONNE, FRANCE UBO, FRANCE UBS, FRANCE UNIV NANTES, FRANCE UNIV PERPIGNAN, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE IFREMER, FRANCE CNRS, FRANCE SI MONTPELLIER ROSCOFF LA TREMBLADE SETE SAINT VINCENT BREST SE PDG-RBE-IHPE PDG-RBE-PFOM PDG-RBE-ASIM PDG-RBE-PHYTNESS PDG-ODE-LITTORAL-LERLR PDG-RBE-LEADNC UM LEMAR MARBEC IHPE EIO ENTROPIE IN WOS Ifremer UPR WOS Ifremer UMR WOS Cotutelle UMR copubli-france copubli-univ-france IF 11.1 TC 1 UR https://archimer.ifremer.fr/doc/00840/95232/102978.pdf https://archimer.ifremer.fr/doc/00840/95232/102979.pdf https://archimer.ifremer.fr/doc/00840/95232/105276.pdf https://archimer.ifremer.fr/doc/00840/95232/105277.pdf LA English DT Article DE ;microbiota;mollusk;immune suppression;T3SS;iron uptake AB Polymicrobial diseases significantly impact the health of humans and animals but remain understudied in natural systems. We recently described the Pacific Oyster Mortality Syndrome (POMS), a polymicrobial disease that impacts oyster production and is prevalent worldwide. Analysis of POMS-infected oysters on the French North Atlantic coast revealed that the disease involves co-infection with the endemic ostreid herpesvirus 1 (OsHV-1) and virulent bacterial species such asVibrio crassostreae. However, it is unknown whether consistentVibriopopulations are associated with POMS in different regions, howVibriocontribute to POMS, and how they interact with the OsHV-1 virus during pathogenesis. We resolved theVibriopopulation structure in oysters from a Mediterranean ecosystem and investigated their functions in POMS development. We find thatVibrio harveyiandVibrio rotiferianusare the predominant species found in OsHV-1-diseased oysters and show that OsHV-1 is necessary to reproduce the partition of theVibriocommunity observed in the field. By characterizing the interspecific interactions between OsHV-1,V. harveyiandV. rotiferianus, we find that onlyV. harveyisynergizes with OsHV-1. When co-infected, OsHV-1 andV. harveyibehave cooperatively by promoting mutual growth and accelerating oyster death.V. harveyishowed high virulence potential in oysters and dampened host cellular defenses, making oysters a more favorable niche for microbe colonization. We next investigated the interactions underlying the co-occurrence of diverseVibriospecies in diseased oysters. We found thatV. harveyiharbors genes responsible for the biosynthesis and uptake of a key siderophore called vibrioferrin. This important resource promotes the growth ofV. rotiferianus, a cheater that efficiently colonizes oysters during POMS without costly investment in host manipulation nor metabolite sharing. By connecting field-based approaches, laboratory infection assays and functional genomics, we have uncovered a web of interdependencies that shape the structure and function of the POMS pathobiota. We showed that cooperative behaviors contribute to synergy between bacterial and viral co-infecting partners. Additional cheating behaviors further shape the polymicrobial consortium. Controlling such behaviors or countering their effects opens new avenues for mitigating polymicrobial diseases. PY 2023 PD OCT SO Proceedings Of The National Academy Of Sciences Of The United States Of America SN 0027-8424 PU National Academy of Sciences VL 120 IS 40 UT 001088032800002 DI 10.1073/pnas.2305195120 ID 95232 ER EF