FN Archimer Export Format
PT J
TI Seasonal patterns in microbial carbon and iron transporter expression in the Southern Ocean
BT 
AF Debeljak, Pavla
   Bayer, Barbara
   Sun, Ying
   Herndl, Gerhard J.
   Obernosterer, Ingrid
AS 1:1,2,3;2:2,4;3:1,5;4:2,6,7;5:1;
FF 1:;2:;3:;4:;5:;
C1 Laboratoire d’Océanographie Microbienne (LOMIC), CNRS, Sorbonne Université, Banyuls/Mer, F-66650, France
   Department of Functional and Evolutionary Ecology, University of Vienna, Djerassiplatz 1, Vienna, 1030, Austria
   SupBiotech, Villejuif, France
   Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Djerassiplatz 1, Vienna, 1030, Austria
   BGI-Qingdao, BGI-Shenzhen, Qingdao, 266555, China
   Department of Marine Microbiology and Biogeochemistry, NIOZ (Royal Netherlands Institute for Sea Research), Den Burg, 1790 AB, The Netherlands
   Vienna Metabolomics Center, University of Vienna, Djerassiplatz 1, Vienna, 1030, Austria
C2 CNRS, FRANCE
   UNIV VIENNA, AUSTRIA
   SUPBIOTECH, FRANCE
   UNIV VIENNA, AUSTRIA
   BGI-QINGDAO, CHINA
   NIOZ, NETHERLANDS
   UNIV VIENNA, AUSTRIA
IF 15.5
TC 0
UR https://archimer.ifremer.fr/doc/00849/96098/104185.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104186.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104187.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104188.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104189.eps
   https://archimer.ifremer.fr/doc/00849/96098/104190.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104191.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104192.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104193.pdf
   https://archimer.ifremer.fr/doc/00849/96098/104194.docx
   https://archimer.ifremer.fr/doc/00849/96098/104195.xlsx
LA English
DT Article
CR MD 205 / SOCLIM
   MOBYDICK-THEMISTO
BO Marion Dufresne
DE ;Prokaryotic membrane transporters;Organic substrate utilization;Iron uptake;Metatranscriptomics;Metaproteomics;Metagenome-assembled genomes;Southern Ocean;Ocean microbiome
AB Background Heterotrophic microbes in the Southern Ocean are challenged by the double constraint of low concentrations of organic carbon (C) and iron (Fe). These essential elements are tightly coupled in cellular processes; however, the prokaryotic requirements of C and Fe under varying environmental settings remain poorly studied. Here, we used a combination of metatranscriptomics and metaproteomics to identify prokaryotic membrane transporters for organic substrates and Fe in naturally iron-fertilized and high-nutrient, low-chlorophyll waters of the Southern Ocean during spring and late summer. Results Pronounced differences in membrane transporter profiles between seasons were observed at both sites, both at the transcript and protein level. When specific compound classes were considered, the two approaches revealed different patterns. At the transcript level, seasonal patterns were only observed for subsets of genes belonging to each transporter category. At the protein level, membrane transporters of organic compounds were relatively more abundant in spring as compared to summer, while the opposite pattern was observed for Fe transporters. These observations suggest an enhanced requirement for organic C in early spring and for Fe in late summer. Mapping transcripts and proteins to 50 metagenomic-assembled genomes revealed distinct taxon-specific seasonal differences pointing to potentially opportunistic clades, such as Pseudomonadales and Nitrincolaceae, and groups with a more restricted repertoire of expressed transporters, such as Alphaproteobacteria and Flavobacteriaceae. Conclusion The combined investigations of C and Fe membrane transporters suggest seasonal changes in the microbial requirements of these elements under different productivity regimes. The taxon-specific acquisition strategies of different forms of C and Fe illustrate how diverse microbes could shape transcript and protein expression profiles at the community level at different seasons. Our results on the C- and Fe-related metabolic capabilities of microbial taxa provide new insights into their potential role in the cycling of C and Fe under varying nutrient regimes in the Southern Ocean. 
PY 2023
PD AUG
SO Microbiome
SN 2049-2618
PU Springer Science and Business Media LLC
VL 11
IS 1
UT 001051143800001
DI 10.1186/s40168-023-01600-3
ID 96098
ER

EF