FN Archimer Export Format
PT J
TI Comparative Analysis of Campylobacter jejuni and C. coli Isolated from Livestock Animals to C. jejuni and C. coli Isolated from Surface Water Using DNA Sequencing and MALDI-TOF
BT 
AF Denis, Martine
   Rose, Valérie
   Nagard, Bérengère
   Thépault, Amandine
   Lucas, Pierrick
   Meunier, Meagan
   Benoit, Fabienne
   Wilhem, Amandine
   Gassilloud, Benoit
   Cauvin, Elodie
   Rincé, Alain
   Gourmelon, Michele
AS 1:1;2:1;3:1;4:1;5:2;6:3;7:3;8:4;9:4;10:3;11:5;12:6;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:PDG-ODE-DYNECO-PELAGOS;
C1 Ploufragan-Plouzané-Niort Laboratory, Hygiene and Quality of Poultry and Pork Products Unit, ANSES (French Agency For Food, Environmental and Occupational Health and Safety), 22440 Ploufragan, France
   Ploufragan-Plouzané-Niort Laboratory, Viral Genetics and Biosafety Unit, ANSES, 22440 Ploufragan, France
   Caen-Saint-Lô Laboratory, Research Department, LABEO, 50000 Saint-Lô, France
   Nancy Laboratory, ANSES, PTF Maldi, 54000 Nancy, France
   Bacterial Communication and Anti-Infectious Strategies Reseach Unit, UNICAEN (Caen Normandie University), UR4312 CBSA, 14000 Caen, France
   ODE-DYNECO-PELAGOS (Department of Oceanography and Ecosystem Dynamics, Coastal Environment Dynamics and Pelagic Ecology Research Unit), IFREMER (French Research Institute for Exploitation of the Sea), 29280 Plouzané, France
C2 ANSES, FRANCE
   ANSES, FRANCE
   LABEO, FRANCE
   ANSES, FRANCE
   UNIV CAEN, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer UPR
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.7
TC 1
UR https://archimer.ifremer.fr/doc/00849/96141/104308.pdf
LA English
DT Article
DE ;Campylobacter;livestock animals;surface water;MLST;cgMLST;structure;MALDI-TOF
AB This study evaluated the contribution of cattle, sheep, poultry and pigs to the contamination of surface water from rivers by Campylobacter jejuni and C. coli using MLST, cgMLST and considered MALDI-TOF MS as an alternative technique. The 263 strains isolated from cattle (n = 61), sheep (n = 42), poultry (n = 65), pigs (n = 60) and surface water (n = 35) were distributed across 115 sequence types (STs), 49 for C. jejuni and 66 for C. coli. Considering MLST data, 14.2%, 11.4% and 2.8% of the surface water strains could be attributed to cattle, poultry and sheep, respectively, none to pigs, and 85.7% were non-attributed. Analysis of cg-MLST data with STRUCTURE indicated that C. jejuni strains from water were predominantly attributed to poultry (93.5%), weakly to sheep (<1%) and 6.3% non-attributed, and that conversely, C. coli strains from water were predominantly non-attributed (94.3%) and 5.7% attributed to poultry. Considering the protein profiles with a threshold of 94% and 97% of similarity, respectively, strains from surface water could be attributed to poultry (31.4% and 17.1%), and to cattle (17.1% and 5.7%); 54.1% and 77.1% were non-attributed. This study confirmed these livestock animals might contribute to the contamination of surface water, with a level of contribution depending on the typing technique and the method of analysis. MALDI-TOF could potentially be an alternative approach for source attribution. 
PY 2023
PD SEP
SO Pathogens
SN 2076-0817
PU MDPI AG
VL 12
IS 9
UT 001076836800001
DI 10.3390/pathogens12091069
ID 96141
ER

EF