FN Archimer Export Format
PT J
TI Circulating MicroRNAs Indicative of Sex and Stress in the European Seabass (Dicentrarchus labrax): Toward the Identification of New Biomarkers
BT 
AF Houdelet, Camille
   Blondeau-Bidet, Eva
   Estevez-Villar, Mathilde
   Mialhe, Xavier
   Hermet, Sophie
   Ruelle, Francois
   Dutto, Gilbert
   Bajek, Aline
   Bobe, Julien
   Geffroy, Benjamin
AS 1:1;2:6;3:3;4:3;5:6;6:1;7:1;8:4;9:5;10:2;
FF 1:PDG-RBE-MARBEC-LAAAS;2:;3:;4:;5:;6:PDG-RBE-MARBEC-LSEA;7:PDG-RBE-MARBEC-LSEA;8:;9:;10:PDG-RBE-MARBEC-LAAAS;
C1 MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Palavas-Les-Flots, France
   MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Montpellier, France
   MGX-Montpellier GenomiX, Institut de Génomique Fonctionnelle, Montpellier, France
   Ecloserie Marine de Gravelines-Ichtus, Voie des Enrochements, F-59820, Gravelines, France
   INRAE, UR1037, Fish Physiology and Genomic laboratory, F-35000, Rennes, France
   MARBEC Univ Montpellier, CNRS, Ifremer, IRD, Montpellier, France
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   ECLOSERIE MARINE GRAVELINES ICHTUS, FRANCE
   INRAE, FRANCE
   UNIV MONTPELLIER, FRANCE
SI PALAVAS
SE PDG-RBE-MARBEC-LAAAS
   PDG-RBE-MARBEC-LSEA
UM MARBEC
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   copubli-france
   copubli-p187
   copubli-univ-france
IF 3
TC 1
UR https://archimer.ifremer.fr/doc/00850/96145/104354.pdf
LA English
DT Article
DE ;Sex differentiation;Cortisol;Glucocorticoid receptor;Fish;Blood plasma;miRNAs
AB MicroRNAs (miRNAs) constitute a new category of biomarkers. Studies on miRNAs in non-mammalian species have drastically increased in the last few years. Here, we explored the use of miRNAs as potential, poorly invasive markers, to identify sex and characterize acute stress in fish. The European seabass (Dicentrarchus labrax) was chosen as a model because of its rapid response to stress and its specific sex determination system, devoid of sexual chromosomes. We performed a small RNA-sequencing analysis in the blood plasma of male and female European seabass (mature and immature) as well as in the blood plasma of juveniles submitted to an acute stress and sampled throughout the recovery period (at 0 h, 0.5 h, 1.5 h and 6 h). In immature individuals, both miR-1388-3p and miR-7132a-5p were up-regulated in females, while miR-499a-5p was more abundant in males. However, no miRNAs were found to be differentially expressed between sexes in the blood plasma of mature individuals. For the acute stress analysis, five miRNAs (miR-155-5p, miR-200a-3p, miR-205-1-5p, miR-143-3p, and miR-223-3p) followed cortisol production over time. All miRNAs identified were tested and validated by RT-qPCR on sequenced samples. A complementary analysis on the 3′UTR sequences of the European seabass allowed to predict potential mRNA targets, some of them being particularly relevant regarding stress regulation, e.g., the glucocorticoid receptor 1 and the mineralocorticoid receptor. The present study provides new avenues and recommendations on the use of miRNAs as biomarkers of sex or stress of the European seabass, with potential application on other fish species. 
PY 2023
PD OCT
SO Marine Biotechnology
SN 1436-2228
PU Springer Science and Business Media LLC
VL 25
IS 5
UT 001048055000001
BP 749
EP 762
DI 10.1007/s10126-023-10237-0
ID 96145
ER

EF