FN Archimer Export Format
PT J
TI Genetic and phenotypic intra-species diversity of alga Tisochrysis lutea reveals original genetic structure and domestication potential
BT 
AF Carrier, Gregory
   Berthelier, Jérémy
   Maupetit, Agathe
   Nicolau, Elodie
   Marbouty, Martial
   Schreiber, Nathalie
   Charrier, Aurelie
   CARCOPINO, Clemence
   Leroi, Laura
   Saint-Jean, Bruno
AS 1:1;2:2;3:1;4:1;5:3;6:1;7:1;8:4;9:4;10:1;
FF 1:PDG-ODE-PHYTOX-GENALG;2:PDG-RBE-BRM-LPBA;3:PDG-RBE-BRM-LPBA;4:PDG-ODE-PHYTOX-GENALG;5:;6:PDG-ODE-PHYTOX-GENALG;7:PDG-ODE-PHYTOX-PHYSALG;8:PDG-IRSI-SEBIMER;9:PDG-IRSI-SEBIMER;10:PDG-ODE-PHYTOX-GENALG;
C1 IFREMER, Unité Physiologie et Toxines des Microalgues Toxine, PHYTOX, Nantes, F-44311, France
   Epigenetics Unit, Okinawa Institute of Science and Technology Graduate University (OIST), Okinawa, 904-0495, Japan
   Institut Pasteur, Unité Régulation Spatiale des Génomes, UMR 3525, CNRS, Paris, F-75015, France
   IFREMER, Service de Bioinformatique de l’Ifremer, SeBiMER, Plouzané, F-29280, France
C2 IFREMER, FRANCE
   OIST, JAPAN
   INSTITUT PASTEUR, FRANCE
   IFREMER, FRANCE
SI NANTES
   BREST
SE PDG-ODE-PHYTOX-GENALG
   PDG-RBE-BRM-LPBA
   PDG-ODE-PHYTOX-PHYSALG
   PDG-IRSI-SEBIMER
IF 2.4
TC 0
UR https://archimer.ifremer.fr/doc/00855/96704/105249.pdf
   https://archimer.ifremer.fr/doc/00855/96704/105250.pdf
   https://archimer.ifremer.fr/doc/00855/96704/105251.xlsx
   https://archimer.ifremer.fr/doc/00855/96704/105252.pdf
   https://archimer.ifremer.fr/doc/00855/96704/105253.docx
LA English
DT Article
DE ;Algae;antioxidant molecules;genetic structure;Haptophyta;intra-species diversity;Tisochrysis lutea
AB Oceanic phytoplankton species are generally composed of many strains, with intra-species diversity consisting of genetic and phenotypic variability. Despite its importance in ecological and biotechnological contexts, this intra-species diversity and variation among strains has been little studied. We investigated the intra-species diversity of the microalga Tisochrysis lutea, a haptophyte of the Isochrysidales order. Inter-strain diversity of T. lutea was studied because of the economic importance of the species as a feed in aquaculture and for antioxidant metabolite production, particularly fucoxanthin and other carotenoids, which have health benefits. We analysed Tara Ocean datasets which revealed that T. lutea was present in the Pacific, Atlantic and Indian Oceans but not in the Arctic or Austral Oceans. We next made phenotypic and genotypic comparisons of 11 strains of T. lutea from worldwide algal collections. All strains were cultivated in the same controlled conditions for one week, and several phenotypic traits were measured, notably antioxidant content. In parallel, the genomes of each strain were sequenced, and genetic variants identified. At the genetic and phenotypic levels, the strains were distinct from each other and our analysis revealed natural trait variations of interest in relation to further exploitation in domestication programmes. A large number of genetic variations were identified among the strains, but no major differences in genome size were observed. Moreover, limited genetic structure was observed among these strains, which could be a consequence of the complex life history of species within the Isochrysidales. Our study provides new knowledge on the intra-species diversity that should be considered in future environmental studies and breeding programmes. Highlights Tisochrysis lutea is found in many parts of the world’s oceans. T. lutea has high inter-strain phenotypic and genomic variation. Genetic structure of strains from culture collections is limited. 
PY 2024
PD FEB
SO European Journal of Phycology
SN 0967-0262
PU Informa UK Limited
VL 59
IS 1
BP 94
EP 111
DI 10.1080/09670262.2023.2249073
ID 96704
ER

EF