FN Archimer Export Format
PT J
TI Processing of matched and mismatched rNMPs in DNA by archaeal ribonucleotide excision repair
BT 
AF Reveil, Maurane
   Chapel, Lucie
   VOURC'H, Blandine
   Bossé, Audrey
   Vialle, Lea
   Brizard, Raphael
   Moalic, Yann
   Jebbar, Mohamed
   Henneke, Ghislaine
AS 1:1;2:1;3:1;4:1;5:1;6:1;7:2;8:2;9:1;
FF 1:PDG-REM-EEP-LMEE;2:PDG-REM-EEP-LMEE;3:PDG-REM-EEP-LMEE;4:PDG-REM-BEEP-LMEE;5:PDG-REM-BEEP-LMEE;6:PDG-REM-BEEP-LMEE;7:;8:;9:PDG-REM-BEEP-LMEE;
C1 Univ Brest, CNRS, Ifremer, UMR6197 Biologie et Ecologie des Ecosystèmes marins Profonds, 1625 Rte de Sainte-Anne 29280 Plouzané, France
   Univ Brest, CNRS, Ifremer, UMR6197 Biologie et Ecologie des Ecosystèmes marins Profonds, 1625 Rte de Sainte-Anne 29280 Plouzané, France
C2 IFREMER, FRANCE
   UBO, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
   PDG-REM-BEEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer UMR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 5.8
TC 0
UR https://archimer.ifremer.fr/doc/00861/97242/106183.pdf
   https://archimer.ifremer.fr/doc/00861/97242/106497.pdf
LA English
DT Article
AB Ribonucleoside monophosphates (rNMPs) are the main non-canonical nucleotides in genomic DNA, and their incorporation can occur as mismatches or matches in vivo. To counteract the mutagenic potential of rNMPs in DNA, all organisms evolved ribonucleotide excision repair (RER), a mechanism initiated by type 2 RNase H. Here, we describe the in vitro reconstitution of matched and mismatched rNMP repair using archaeal RER enzymes. Our data suggest two types of RER pathways, including the classical flap RER and a back-up RER with the order of reactions changed for Fen1 and Pols. The genomic rNMP level in RER-deficient or PolB-deficient archaeal cells along with in vitro reconstitution of RER suggest an in vivo role of PolD in RER. Our results provide insights into how matched and mismatched rNMPs may be processed by RER. 
PY 2023
PD DEC
SO Iscience
SN 2589-0042
PU Elsevier BV
VL 26
IS 12
UT 001131634800001
DI 10.1016/j.isci.2023.108479
ID 97242
ER

EF