FN Archimer Export Format
PT J
TI Tissue Distribution and Metabolization of Ciguatoxins in an Herbivorous Fish following Experimental Dietary Exposure to Gambierdiscus polynesiensis
BT 
AF Clausing, Rachel J.
   Ben Gharbia, Hela
   Sdiri, Khalil
   Sibat, Manoella
   Rañada-Mestizo, Ma. Llorina
   Lavenu, Laura
   Hess, Philipp
   Chinain, Mireille
   Bottein, Marie-Yasmine Dechraoui
AS 1:1,2;2:3;3:3;4:4;5:5;6:3;7:4;8:6;9:7;
FF 1:;2:;3:;4:PDG-ODE-PHYTOX-METALG;5:;6:;7:PDG-ODE-PHYTOX;8:;9:;
C1 Dipartimento di Scienze della Terra dell’Ambiente e della Vita, Università degli Studi di Genova, 16132 Genova, Italy
   Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095, USA
   IAEA Marine Environment Laboratories, International Atomic Energy Agency, 98000 Monaco, Monaco
   Ifremer, ODE/PHYTOX/METALG, Rue de l’île d’Yeu, F-44300 Nantes, France
   IAEA Collaborating Center on Harmful Algal Bloom (HAB) Studies, Chemistry Research Section, Department of Science and Technology, Philippine Nuclear Research Institute (DOST-PNRI), Diliman, Quezon City 1101, Philippines
   Laboratoire des Biotoxines Marines, UMR 241 EIO, Institut Louis Malardé, BP 30, Papeete-Tahiti 98713, French Polynesia
   Université Côte d’Azur, CNRS, ECOSEAS, UMR7035, Parc Valrose, CEDEX 2, 06103 Nice, France
C2 UNIV GENOVA, ITALY
   UNIV CALIF LOS ANGELES, USA
   IAEA, MONACO
   IFREMER, FRANCE
   IAEA, PHILIPPINES
   INST LOUIS MALARDE, FRANCE
   UNIV COTE D’AZUR, FRANCE
SI NANTES
SE PDG-ODE-PHYTOX-METALG
   PDG-ODE-PHYTOX
UM EIO
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 5.4
TC 0
UR https://archimer.ifremer.fr/doc/00868/98004/107254.pdf
   https://archimer.ifremer.fr/doc/00868/98004/107255.zip
LA English
DT Article
DE ;ciguatoxins;ciguatera poisoning (CP);trophic transfer;metabolism;biotransformation;bioaccumulation;tissue distribution;Gambierdiscus polynesiensis;reef fish;liquid chromatography-tandem mass spectrometry
AB Ciguatoxins (CTXs), potent neurotoxins produced by dinoflagellates of the genera Gambierdiscus and Fukuyoa, accumulate in commonly consumed fish species, causing human ciguatera poisoning. Field collections of Pacific reef fish reveal that consumed CTXs undergo oxidative biotransformations, resulting in numerous, often toxified analogs. Following our study showing rapid CTX accumulation in flesh of an herbivorous fish, we used the same laboratory model to examine the tissue distribution and metabolization of Pacific CTXs following long-term dietary exposure. Naso brevirostris consumed cells of Gambierdiscus polynesiensis in a gel food matrix over 16 weeks at a constant dose rate of 0.36 ng CTX3C equiv g−1 fish d−1. CTX toxicity determination of fish tissues showed CTX activity in all tissues of exposed fish (eight tissues plus the carcass), with the highest concentrations in the spleen. Muscle tissue retained the largest proportion of CTXs, with 44% of the total tissue burden. Moreover, relative to our previous study, we found that larger fish with slower growth rates assimilated a higher proportion of ingested toxin in their flesh (13% vs. 2%). Analysis of muscle extracts revealed the presence of CTX3C and CTX3B as well as a biotransformed product showing the m/z transitions of 2,3-dihydroxyCTX3C. This is the first experimental evidence of oxidative transformation of an algal CTX in a model consumer and known vector of CTX into the fish food web. These findings that the flesh intended for human consumption carries the majority of the toxin load, and that growth rates can influence the relationship between exposure and accumulation, have significant implications in risk assessment and the development of regulatory measures aimed at ensuring seafood safety. 
PY 2024
PD JAN
SO Marine Drugs
SN 1660-3397
PU MDPI AG
VL 22
IS 1
UT 001152770400001
DI 10.3390/md22010014
ID 98004
ER

EF