FN Archimer Export Format
PT J
TI Hypometabolism to survive the long polar night and subsequent successful return to light in the diatom Fragilariopsis cylindrus
BT 
AF Joli, Nathalie
   Concia, Lorenzo
   Mocaer, Karel
   Guterman, Julie
   Laude, Juliette
   Guerin, Sebastien
   Sciandra, Theo
   Bruyant, Flavienne
   Ait‐Mohamed, Ouardia
   Beguin, Marine
   Forget, Marie‐Helene
   Bourbousse, Clara
   Lacour, Thomas
   Bailleul, Benjamin
   Nef, Charlotte
   Savoie, Mireille
   Tremblay, Jean‐Eric
   Campbell, Douglas A.
   Lavaud, Johann
   Schwab, Yannick
   Babin, Marcel
   Bowler, Chris
AS 1:1;2:1;3:2;4:1;5:1;6:3;7:1,3;8:3;9:1;10:3;11:3;12:1;13:4;14:5;15:1;16:6;17:7;18:6;19:3,8;20:9;21:3;22:1;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:PDG-ODE-PHYTOX-PHYSALG;14:;15:PDG-RBE-BRM-LPBA;16:;17:;18:;19:;20:;21:;22:;
C1 Institut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM PSL Université Paris 75005 Paris ,France
   Cell Biology and Biophysics Unit European Molecular Biology Laboratory (EMBL) & Collaboration for Joint PhD Degree between the European Molecular Biology Laboratory and the Heidelberg University, Faculty of Biosciences 69117 Heidelberg, Germany
   Takuvik International Research Laboratory, Université Laval (Canada) & CNRS (France), Département de Biologie and Québec‐Océan Université Laval Québec QC G1V 0A6 ,Canada
   Laboratoire PHYSiologie des micro ALGues (PDG‐ODE‐PHYTOX‐PHYSALG) Centre Atlantique 44 311 Nantes ,France
   Laboratory of Chloroplast Biology and Light Sensing in Microalgae, Institut de Biologie Physico Chimique, CNRS Sorbonne Université Paris 75005 ,France
   Département de Biologie Université Laval Québec QC G1V 0A6 ,Canada
   Mount Allison University Sackville NB E4L 1H3, Canada
   UMR 6539 LEMAR‐Laboratory of Environmental Marine Sciences, CNRS/Univ Brest/Ifremer/IRD IUEM‐Institut Européen de la Mer, Technopôle Brest‐Iroise rue Dumont d'Urville 29280 Plouzané ,France
   Cell Biology and Biophysics Unit and Electron Microscopy Core Facility European Molecular Biology Laboratory (EMBL) 69117 Heidelberg ,Germany
C2 ENS, FRANCE
   UNIV HEIDELBERG, GERMANY
   UNIV LAVAL, CANADA
   IFREMER, FRANCE
   UNIV SORBONNE, FRANCE
   UNIV LAVAL, CANADA
   UNIV MOUNT ALLISON, CANADA
   UBO, FRANCE
   EMBL, GERMANY
SI NANTES
   BREST
SE PDG-ODE-PHYTOX-PHYSALG
   PDG-RBE-BRM-LPBA
   CNRS
UM LEMAR
IF 9.4
TC 0
UR https://archimer.ifremer.fr/doc/00868/98039/107324.pdf
   https://archimer.ifremer.fr/doc/00868/98039/107326.gff
   https://archimer.ifremer.fr/doc/00868/98039/107327.gff3
   https://archimer.ifremer.fr/doc/00868/98039/107328.pdf
   https://archimer.ifremer.fr/doc/00868/98039/107329.xlsx
   https://archimer.ifremer.fr/doc/00868/98039/107330.avi
   https://archimer.ifremer.fr/doc/00868/98039/107331.avi
   https://archimer.ifremer.fr/doc/00868/98039/107332.avi
   https://archimer.ifremer.fr/doc/00868/98039/107333.avi
   https://archimer.ifremer.fr/doc/00868/98039/107334.avi
   https://archimer.ifremer.fr/doc/00868/98039/107335.avi
LA English
DT Article
DE ;autophagy;diatom;energy homeostasis;Fragilariopsis cylindrus;polar night;quiescence
AB Summary Diatoms, the main eukaryotic phytoplankton of the polar marine regions, are essential for the maintenance of food chains specific to Arctic and Antarctic ecosystems, and are experiencing major disturbances under current climate change. As such, it is fundamental to understand the physiological mechanisms and associated molecular basis of their endurance during the long polar night. Here, using the polar diatom Fragilariopsis cylindrus, we report an integrative analysis combining transcriptomic, microscopic and biochemical approaches to shed light on the strategies used to survive the polar night. We reveal that in prolonged darkness, diatom cells enter a state of quiescence with reduced metabolic and transcriptional activity, during which no cell division occurs. We propose that minimal energy is provided by respiration and degradation of protein, carbohydrate and lipid stores and that homeostasis is maintained by autophagy in prolonged darkness. We also report internal structural changes that manifest the morphological acclimation of cells to darkness, including the appearance of a large vacuole. Our results further show that immediately following a return to light, diatom cells are able to use photoprotective mechanisms and rapidly resume photosynthesis, demonstrating the remarkable robustness of polar diatoms to prolonged darkness at low temperature. 
PY 2024
PD MAR
SO New Phytologist
SN 0028-646X
PU Wiley
VL 241
IS 5
BP 2193
EP 2208
DI 10.1111/nph.19387
ID 98039
ER

EF