FN Archimer Export Format
PT J
TI Co-infection of two eukaryotic pathogens within clam populations in Arcachon Bay
BT 
AF Itoïz, Sarah
   Mouronvalle, Clara
   Perennou, Morgan
   Chailler, Elisa
   Smits, Morgan
   Derelle, Evelyne
   Metz, Sebastian
   Le Goïc, Nelly
   Bidault, Adeline
   de Montaudouin, Xavier
   Arzul, Isabelle
   Soudant, Philippe
   Chambouvet, Aurélie
AS 1:1;2:1,2;3:6;4:3;5:1;6:1;7:3;8:6;9:1;10:4;11:5;12:6;13:3;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:PDG-RBE-ASIM;12:;13:;
C1 Univ Brest, CNRS, IRD, Ifremer, LEMAR, Plouzané, France
   EPHE, PSL Research University, UPVD, CNRS, USR CRIOBE, Perpignan, France
   CNRS, UMR7144 Adaptation et Diversité en Milieu Marin, Ecology of Marine Plankton (ECOMAP), Station Biologique de Roscoff SBR, Sorbonne University, Roscoff, France
   Univ. Bordeaux, CNRS, Bordeaux INP, EPOC, UMR, Station Marine d’Arcachon, Arcachon, France
   Ifremer, ASIM Adaptation et Santé des Invertébrés Marins, La Tremblade, France
   Univ Brest, CNRS, IRD, Ifremer, LEMAR, Plouzané, France
C2 UBO, FRANCE
   EPHE, FRANCE
   CNRS, FRANCE
   UNIV BORDEAUX, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
SI LA TREMBLADE
SE PDG-RBE-ASIM
UM LEMAR
IN WOS Ifremer UPR
   WOS Cotutelle UMR
   DOAJ
   copubli-france
   copubli-univ-france
IF 5.2
TC 0
UR https://archimer.ifremer.fr/doc/00870/98154/107477.pdf
   https://archimer.ifremer.fr/doc/00870/98154/107478.zip
LA English
DT Article
BO Planula IV
DE ;Perkinsosis;parasite;multiple-infection;co-infection;Manila clam;organ distribution
AB The parasitic species Perkinsus olseni (= atlanticus) (Perkinsea, Alveolata) infects a wide range of mollusc species and is responsible for mortality events and economic losses in the aquaculture industry and fisheries worldwide. Thus far, most studies conducted in this field have approached the problem from a “one parasite-one disease” perspective, notably with regards to commercially relevant clam species, while the impact of other Perkinsus species should also be considered as it could play a key role in the disease phenotype and dynamics. Co-infection of P. olseni and P. chesapeaki has already been sporadically described in Manila clam populations in Europe. Here, we describe for the first time the parasitic distribution of two Perkinsus species, P. olseni and P. chesapeaki, in individual clam organs and in five different locations across Arcachon Bay (France), using simultaneous in situ detection by quantitative PCR (qPCR) duplex methodology. We show that P. olseni single-infection largely dominated prevalence (46–84%) with high intensities of infection (7.2 to 8.5 log-nb of copies. g−1of wet tissue of Manila clam) depending on location, suggesting that infection is driven by the abiotic characteristics of stations and physiological states of the host. Conversely, single P. chesapeaki infections were observed in only two sampling stations, Ile aux Oiseaux and Gujan, with low prevalences 2 and 14%, respectively. Interestingly, the co-infection by both Perkinsus spp., ranging in prevalence from 12 to 34%, was distributed across four stations of Arcachon Bay, and was detected in one or two organs maximum. Within these co-infected organs, P. olseni largely dominated the global parasitic load. Hence, the co-infection dynamics between P. olseni and P. chesapeaki may rely on a facilitating role of P. olseni in developing a primary infection which in turn may help P. chesapeaki infect R. philippinarum as a reservoir for a preferred host. This ecological study demonstrates that the detection and quantification of both parasitic species, P. olseni and P. chesapeaki, is essential and timely in resolving cryptic infections and their consequences on individual hosts and clam populations. 
PY 2024
PD JAN
SO Frontiers In Microbiology
SN 1664-302X
PU Frontiers Media SA
VL 14
UT 001152769900001
DI 10.3389/fmicb.2023.1250947
ID 98154
ER

EF